ID   C16AA_PARBF             Reviewed;         613 AA.
AC   Q45882;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Pesticidal crystal-like protein Cry16Aa;
DE   AltName: Full=Cbm71 mosquitocidal toxin;
DE   AltName: Full=Insecticidal toxin CryXVIA(a);
GN   Name=cry16Aa; Synonyms=cbm71, cryXVIA(a);
OS   Paraclostridium bifermentans (Clostridium bifermentans).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Paraclostridium.
OX   NCBI_TaxID=1490;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15 AND
RP   374-388.
RC   STRAIN=CH18 / subsp. Malaysia;
RX   PubMed=8655486; DOI=10.1128/jb.178.11.3099-3105.1996;
RA   Barloy F., Delecluse A., Nicolas L., Lecadet M.-M.;
RT   "Cloning and expression of the first anaerobic toxin gene from Clostridium
RT   bifermentans subsp. malaysia, encoding a new mosquitocidal protein with
RT   homologies to Bacillus thuringiensis delta-endotoxins.";
RL   J. Bacteriol. 178:3099-3105(1996).
CC   -!- FUNCTION: Toxin active on mosquito larvae of the species Aedes aegypti,
CC       Culex pipiens and Anopheles stephensi.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DEVELOPMENTAL STAGE: Produced during sporulation. The expression
CC       decreases considerably with cell lysis.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; X94146; CAA63860.1; -; Genomic_DNA.
DR   PIR; JC6033; JC6033.
DR   AlphaFoldDB; Q45882; -.
DR   SMR; Q45882; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Sporulation; Toxin; Virulence.
FT   CHAIN           1..613
FT                   /note="Pesticidal crystal-like protein Cry16Aa"
FT                   /id="PRO_0000174089"
FT   CONFLICT        9
FT                   /note="H -> Q (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   613 AA;  71173 MW;  410EEFD9C601CEE5 CRC64;
     MNTNIFSTHL EFSKGVASVF KVIDTIHNIS KNNNFNNILT QDFIIDTILS ILWEDPNENE
     IFSSMIEDGE TITNKNLSAQ TKEGLLLNSN SFGLKFKYYN NAFRSWIDNY NPTSIDDVVY
     RFKDVNSICE NNINEFKVKN YEVTVLPIYM QIANLHLLLL RDGMIYGDAW NLYRELGFSD
     QDSFYNHVLD KTKFYINDCL NYYNTGLSNL KLDPNNSWID ITRYCRFMTF YILDMISICP
     IYDTKVYDKP INMQTLTRKV YSDPVNFIDE NIPISEYEKM YNISPELFST LFSISFYTNK
     SGNKFLNGHV NRHVGTDLNY NGLRETHYGN YGSNYEVESM AFDDIKAYSN NYFNNTQNNN
     PTSVKSIKFL ITKNNDEWIY GEPDSSNIDF TRNIQGYLSN LNNESYTHSL SDMILANNDK
     IQINIDTPHS YSYSWIYKGI EDTNYISDKL INQIPLVKEV KLKSRHYSEI SVIKGPGFTG
     GDLILSKVHK PANQIPAQYM KNKITIPIKT KFPAGSQDFK VRLCYASNHD IGLIRLIAGS
     KYITTNIQQT FNTTENNPSL IYDDFKYFNF NETLSITSSG IDELYLEFYY SYTDGNFEDF
     PKLSIPYTRN YSC