ID   ACAP_LACLM              Reviewed;         489 AA.
AC   A2RL65;
DT   20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Aspartate/glutamate permease AcaP {ECO:0000305};
GN   Name=acaP {ECO:0000303|PubMed:23144255};
GN   OrderedLocusNames=llmg_1452 {ECO:0000312|EMBL:CAL98032.1};
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MG1363;
RX   PubMed=23144255; DOI=10.1128/jb.01948-12;
RA   Trip H., Mulder N.L., Lolkema J.S.;
RT   "Cloning, expression, and functional characterization of secondary amino
RT   acid transporters of Lactococcus lactis.";
RL   J. Bacteriol. 195:340-350(2013).
CC   -!- FUNCTION: Involved in aspartate and glutamate uptake. Plays no
CC       significant role in the excretion of accumulated glutamate.
CC       {ECO:0000269|PubMed:23144255}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Disruption of the gene leads to strong reduction
CC       of aspartate uptake, but only marginally affects glutamate uptake.
CC       {ECO:0000269|PubMed:23144255}.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family.
CC       {ECO:0000305}.
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DR   EMBL; AM406671; CAL98032.1; -; Genomic_DNA.
DR   RefSeq; WP_011835304.1; NZ_WJVF01000002.1.
DR   AlphaFoldDB; A2RL65; -.
DR   SMR; A2RL65; -.
DR   STRING; 416870.llmg_1452; -.
DR   EnsemblBacteria; CAL98032; CAL98032; llmg_1452.
DR   KEGG; llm:llmg_1452; -.
DR   eggNOG; COG0531; Bacteria.
DR   HOGENOM; CLU_020854_0_1_9; -.
DR   OMA; IVMPMRY; -.
DR   PhylomeDB; A2RL65; -.
DR   BioCyc; LLAC416870:LLMG_RS07330-MON; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR002293; AA/rel_permease1.
DR   Pfam; PF13520; AA_permease_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..489
FT                   /note="Aspartate/glutamate permease AcaP"
FT                   /id="PRO_0000442544"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        290..310
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        413..433
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        449..469
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   489 AA;  53950 MW;  A04D8BD83FBA0FE5 CRC64;
     MDTKKIRWFT VAFIAFNMVW GMGNVVNNFA QQGITVVTSW LLILALYFIP YALIVGQLCS
     TFKDSKGGVS SWVENTSTKR LAYYAAWTYW VVHIPYLAQK PQAILIAFGW VGQGNGNLVS
     QMSMTAVALI SLAIFLAFLW LSTKGLNTLK VIGGLAGTAM FVMSLLFIVM AIGAPFIAKD
     FHIATPDMGN IKTYIPKFDF SYFTTISMLV FAVGGAEKIS PYVNQTKNPA KEFPRGMFLL
     AGMVGICAVL GSIAMGMIFS SGNLPKDLMA NGAYAAFQIL GQHFGVGNFL MIVYALTNGV
     GQIAALAFSI DAPLRILLAD ADPEYVPAWL RKKTNKGTLK NGYTLTGILV SIIILLPLLG
     IGDMNELVKW LTNLNSVVMP MRYLWVFFAF IMLNRAVKHF QSEYKFIKQK RLAMIAGAWC
     FLFTLIACVL GMVPKLDYAA NPSAWWFQLA SNILTPIVLI LLGMLLPFIA RREQRKATSL
     DLDTIVTPD