C19AA_DANRE
ID C19AA_DANRE Reviewed; 509 AA.
AC O42145;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Aromatase;
DE EC=1.14.14.14 {ECO:0000250|UniProtKB:P11511};
DE AltName: Full=CYPXIX;
DE AltName: Full=Cytochrome P-450AROM;
DE AltName: Full=Cytochrome P450 19A1a;
DE AltName: Full=Estrogen synthase;
GN Name=cyp19a1a; Synonyms=cyp19, cyp19a;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Ovary;
RA Bauer M.P., Goetz F.W.;
RT "Isolation and characterization of a zebrafish (Danio rerio) aromatase
RT cDNA.";
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of aromatic C18 estrogens from C19
CC androgens. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 O2 + 3 reduced [NADPH--hemoprotein reductase] + testosterone
CC = 17beta-estradiol + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--
CC hemoprotein reductase]; Xref=Rhea:RHEA:38191, Rhea:RHEA-COMP:11964,
CC Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:16469,
CC ChEBI:CHEBI:17347, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC EC=1.14.14.14; Evidence={ECO:0000250|UniProtKB:P11511};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=androst-4-ene-3,17-dione + 3 O2 + 3 reduced [NADPH--
CC hemoprotein reductase] = estrone + formate + 4 H(+) + 4 H2O + 3
CC oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:38195,
CC Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740,
CC ChEBI:CHEBI:16422, ChEBI:CHEBI:17263, ChEBI:CHEBI:57618,
CC ChEBI:CHEBI:58210; EC=1.14.14.14;
CC Evidence={ECO:0000250|UniProtKB:P11511};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AF004521; AAB65788.1; -; mRNA.
DR AlphaFoldDB; O42145; -.
DR SMR; O42145; -.
DR STRING; 7955.ENSDARP00000111604; -.
DR PaxDb; O42145; -.
DR ZFIN; ZDB-GENE-990415-43; cyp19a1a.
DR eggNOG; KOG0157; Eukaryota.
DR InParanoid; O42145; -.
DR Reactome; R-DRE-193144; Estrogen biosynthesis.
DR Reactome; R-DRE-211976; Endogenous sterols.
DR PRO; PR:O42145; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070330; F:aromatase activity; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0019099; P:female germ-line sex determination; IMP:ZFIN.
DR GO; GO:0008585; P:female gonad development; IMP:ZFIN.
DR GO; GO:0030237; P:female sex determination; IMP:ZFIN.
DR GO; GO:0046660; P:female sex differentiation; IMP:ZFIN.
DR GO; GO:0019100; P:male germ-line sex determination; IMP:ZFIN.
DR GO; GO:0001541; P:ovarian follicle development; IMP:ZFIN.
DR GO; GO:1904076; P:regulation of estrogen biosynthetic process; IMP:ZFIN.
DR GO; GO:0032355; P:response to estradiol; IDA:ZFIN.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:ZFIN.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..509
FT /note="Aromatase"
FT /id="PRO_0000051967"
FT BINDING 456
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 509 AA; 57354 MW; F04532DEA8FDB628 CRC64;
MAGDLLQPCG MKPVRLGEAV VDLLIQRAHN GTERAQDNAC GATATILLLL LCLLLAIRHH
RPHKSHIPGP SFFFGLGPVV SYCRFIWSGI GTASNYYNSK YGDIVRVWIN GEETLILSRS
SAVYHVLRKS LYTSRFGSKL GLQCIGMHEQ GIIFNSNVAL WKKVRAFYAK ALTGPGLQRT
MEICTTSTNS HLDDLSQLTD AQGQLDILNL LRCIVVDVSN RLFLGVPLNE HDLLQKIHKY
FDTWQTVLIK PDVYFRLDWL HRKHKRDAQE LQDAITALIE QKKVQLAHAE KLDHLDFTAE
LIFAQSHGEL SAENVRQCVL EMVIAAPDTL SISLFFMLLL LKQNPDVELK ILQEMDSVLA
GQSLQHSHLS KLQILESFIN ESLRFHPVVD FTMRRALDDD VIEGYNVKKG TNIILNVGRM
HRSEFFSKPN QFSLDNFHKN VPSRFFQPFG SGPRSCVGKH IAMVMMKSIL VALLSRFSVC
PMKACTVENI PQTNNLSQQP VEEPSSLSV
