TRPE_RHIME
ID TRPE_RHIME Reviewed; 729 AA.
AC P15395;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Anthranilate synthase;
DE EC=4.1.3.27;
DE Includes:
DE RecName: Full=Glutamine amidotransferase;
GN Name=trpE(G); OrderedLocusNames=R02387; ORFNames=SMc02725;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2656657; DOI=10.1128/jb.171.6.3471-3478.1989;
RA Bae Y.M., Holmgren E., Crawford I.P.;
RT "Rhizobium meliloti anthranilate synthase gene: cloning, sequence, and
RT expression in Escherichia coli.";
RL J. Bacteriol. 171:3471-3478(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chorismate + L-glutamine = anthranilate + H(+) + L-glutamate +
CC pyruvate; Xref=Rhea:RHEA:21732, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16567, ChEBI:CHEBI:29748, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359; EC=4.1.3.27;
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 1/5.
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DR EMBL; M22983; AAA26370.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC46966.1; -; Genomic_DNA.
DR PIR; B44515; A30904.
DR RefSeq; NP_386493.1; NC_003047.1.
DR RefSeq; WP_010969900.1; NC_003047.1.
DR AlphaFoldDB; P15395; -.
DR SMR; P15395; -.
DR STRING; 266834.SMc02725; -.
DR MEROPS; C26.955; -.
DR EnsemblBacteria; CAC46966; CAC46966; SMc02725.
DR GeneID; 61603846; -.
DR KEGG; sme:SMc02725; -.
DR PATRIC; fig|266834.11.peg.3871; -.
DR eggNOG; COG0147; Bacteria.
DR eggNOG; COG0512; Bacteria.
DR HOGENOM; CLU_006493_3_0_5; -.
DR OMA; AYRSFMN; -.
DR UniPathway; UPA00035; UER00040.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0004049; F:anthranilate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01743; GATase1_Anthranilate_Synthase; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.60.120.10; -; 1.
DR InterPro; IPR005801; ADC_synthase.
DR InterPro; IPR019999; Anth_synth_I-like.
DR InterPro; IPR006805; Anth_synth_I_N.
DR InterPro; IPR015890; Chorismate_C.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR017926; GATASE.
DR InterPro; IPR010112; TrpE-G_bact.
DR InterPro; IPR006221; TrpG/PapA_dom.
DR PANTHER; PTHR11236; PTHR11236; 1.
DR Pfam; PF04715; Anth_synt_I_N; 1.
DR Pfam; PF00425; Chorismate_bind; 1.
DR Pfam; PF00117; GATase; 1.
DR PIRSF; PIRSF036934; TrpE-G; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR SUPFAM; SSF56322; SSF56322; 1.
DR TIGRFAMs; TIGR01815; TrpE-clade3; 1.
DR TIGRFAMs; TIGR00566; trpG_papA; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 4: Predicted;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis;
KW Glutamine amidotransferase; Lyase; Reference proteome;
KW Tryptophan biosynthesis.
FT CHAIN 1..729
FT /note="Anthranilate synthase"
FT /id="PRO_0000056868"
FT DOMAIN 530..726
FT /note="Glutamine amidotransferase type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT ACT_SITE 607
FT /note="Nucleophile; for GATase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT ACT_SITE 697
FT /note="For GATase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT ACT_SITE 699
FT /note="For GATase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00605"
FT BINDING 580..582
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250|UniProtKB:P00900"
FT BINDING 611
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250|UniProtKB:P00900"
FT BINDING 658..659
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250|UniProtKB:P00900"
SQ SEQUENCE 729 AA; 80505 MW; F16F10A90E3846E1 CRC64;
MAAVILEDGA ESYTTKGGIV VTRRRREASY SDAIAGYVDR LDERRGAVFS SNYEYPGRYT
RWDTAVVDPP LAISSFGRSL WIEAYNERGE VLLALIAEDL KSVADITLGS LAARRLDLTI
NEPDRVFTEE ERSKMPTVFT VLRAVTNLFH SEEDSNLGLY GAFGYDLAFQ FDAIELKLSR
PDDQRDMVLF LPDEILVVDH YAAKAWIDRY DFARENLSTE GKAADIAPEP FRSVDSIPPH
GDHRPGEYAE LVVKAKESFR RGDLFEVVPG QKFYERCESR PSEISNRLKA INPSPYSFFI
NLGNQEYLVG ASPEMFVRVS GRRIETCPIS GTIKRGDDPI ADSEQILKLL NSKKDESELT
MCSDVDRNDK SRVCVPGSVK VIGRRQIEMY SRLIHTVDHI EGRLRDDMDA FDGFLSHAWA
VTVTGAPKLW AMRFIESHEK SPRAWYGGAI GMVGFNGDMN TGLTLRTIRI KDGIAEVRAG
ATLLYDSNPE EEEAETELKA SAMIAAIRDA KSANSAKSAR DVAAVGAGVS ILLVDHEDSF
VHTLANYFRQ TGASVTTVRT PVAEEIFDRV KPDLVVLSPG PGTPKDFDCK ATIKKARARD
LPIFGVCLGL QALAEAYGGD LRQLAIPMHG KPSRIRVLEP GIVFSGLGKE VTVGRYHSIF
ADPSNLPREF VITAESEDGT IMGIEHSKEP VAAVQFHPES IMTLGGDAGM RMIENVVAHL
AKRAKTKAA
