TRPF_BACSU
ID TRPF_BACSU Reviewed; 215 AA.
AC P20167;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 3.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase;
DE Short=PRAI;
DE EC=5.3.1.24;
GN Name=trpF; OrderedLocusNames=BSU22650;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3924737; DOI=10.1016/0378-1119(85)90125-8;
RA Henner D.J., Band L., Shimotsu H.;
RT "Nucleotide sequence of the Bacillus subtilis tryptophan operon.";
RL Gene 34:169-177(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=10568751; DOI=10.1101/gr.9.11.1116;
RA Medigue C., Rose M., Viari A., Danchin A.;
RT "Detecting and analyzing DNA sequencing errors: toward a higher quality of
RT the Bacillus subtilis genome sequence.";
RL Genome Res. 9:1116-1127(1999).
RN [4]
RP SEQUENCE REVISION TO 92 AND 147.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-
CC carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate;
CC Xref=Rhea:RHEA:21540, ChEBI:CHEBI:18277, ChEBI:CHEBI:58613;
CC EC=5.3.1.24;
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 3/5.
CC -!- SIMILARITY: Belongs to the TrpF family. {ECO:0000305}.
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DR EMBL; M80245; AAA20864.1; -; Genomic_DNA.
DR EMBL; K01391; AAA22868.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14181.3; -; Genomic_DNA.
DR PIR; D22794; D22794.
DR RefSeq; NP_390146.3; NC_000964.3.
DR RefSeq; WP_004398742.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P20167; -.
DR SMR; P20167; -.
DR STRING; 224308.BSU22650; -.
DR PaxDb; P20167; -.
DR PRIDE; P20167; -.
DR DNASU; 939006; -.
DR EnsemblBacteria; CAB14181; CAB14181; BSU_22650.
DR GeneID; 939006; -.
DR KEGG; bsu:BSU22650; -.
DR eggNOG; COG0135; Bacteria.
DR InParanoid; P20167; -.
DR OMA; FYAKSPR; -.
DR PhylomeDB; P20167; -.
DR BioCyc; BSUB:BSU22650-MON; -.
DR UniPathway; UPA00035; UER00042.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0004640; F:phosphoribosylanthranilate isomerase activity; IBA:GO_Central.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IBA:GO_Central.
DR CDD; cd00405; PRAI; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00135; PRAI; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR001240; PRAI_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR InterPro; IPR044643; TrpF_fam.
DR PANTHER; PTHR42894; PTHR42894; 1.
DR Pfam; PF00697; PRAI; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Isomerase;
KW Reference proteome; Tryptophan biosynthesis.
FT CHAIN 1..215
FT /note="N-(5'-phosphoribosyl)anthranilate isomerase"
FT /id="PRO_0000154347"
FT CONFLICT 15
FT /note="K -> Q (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="E -> D (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 36
FT /note="K -> Y (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="E -> Q (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="N -> S (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 52
FT /note="R -> C (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 74..77
FT /note="KSLK -> EDLN (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="K -> E (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 93
FT /note="A -> D (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="K -> M (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 100
FT /note="G -> D (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 110..111
FT /note="QD -> HE (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 123
FT /note="N -> D (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="S -> A (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 147
FT /note="E -> D (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 171
FT /note="S -> T (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 174..178
FT /note="RLLKW -> DLMKY (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="E -> A (in Ref. 1; AAA20864/AAA22868)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 215 AA; 24041 MW; 0153C28BA53DC9ED CRC64;
MKKPALKYCG IRSLKDLQLA AESQADYLGF IFAESKRKVS PEDVKKWLNQ VRVEKQVAGV
FVNESIETMS RIAKSLKLDV IQLHGDEKPA DAAALRKLTG CEIWKALHHQ DNTTQEIARF
KDNVDGFVID SSVKGSRGGT GVAFSWECVP EYQQAAIGKR CFIAGGVNPD SITRLLKWQP
EGIDLASGIE KNGQKDQNLM RLLEERMNRY VSISE
