TRPM5_HUMAN
ID TRPM5_HUMAN Reviewed; 1165 AA.
AC Q9NZQ8; A6NHS0; Q52LU2; Q9NY34;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Transient receptor potential cation channel subfamily M member 5;
DE AltName: Full=Long transient receptor potential channel 5;
DE Short=LTrpC-5;
DE Short=LTrpC5;
DE AltName: Full=MLSN1- and TRP-related gene 1 protein;
GN Name=TRPM5 {ECO:0000312|HGNC:HGNC:14323};
GN Synonyms=LTRPC5 {ECO:0000312|EMBL:CAB66342.1},
GN MTR1 {ECO:0000312|EMBL:AAF26288.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAF26288.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3),
RP AND TISSUE SPECIFICITY.
RX PubMed=10607831; DOI=10.1093/hmg/9.2.203;
RA Prawitt D., Enklaar T., Klemm G., Gaertner B., Spangenberg C.,
RA Winterpacht A., Higgins M., Pelletier J., Zabel B.;
RT "Identification and characterization of MTR1, a novel gene with homology to
RT melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical
RT region on chromosome 11p15.5 and showing allele-specific expression.";
RL Hum. Mol. Genet. 9:203-216(2000).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAH93787.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ALA-254.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4] {ECO:0000305, ECO:0000312|EMBL:CAB66342.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 9-1165 (ISOFORM 2).
RA Paulsen M., El-Maarri O., Engemann S., Franck O., Stroedicke M.,
RA Davies K.R., Bowden L.M., Reinhardt R., Reik W., Harteneck C., Walter J.;
RT "Comparative sequence analysis and characterization of the imprinting
RT cluster on the human chromosome 11p15.5 and distal mouse chromosome 7.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=14634208; DOI=10.1073/pnas.2334624100;
RA Prawitt D., Monteilh-Zoller M.K., Brixel L., Spangenberg C., Zabel B.,
RA Fleig A., Penner R.;
RT "TRPM5 is a transient Ca2+-activated cation channel responding to rapid
RT changes in [Ca2+]i.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:15166-15171(2003).
CC -!- FUNCTION: Voltage-modulated Ca(2+)-activated, monovalent cation channel
CC (VCAM) that mediates a transient membrane depolarization and plays a
CC central role in taste transduction. Monovalent-specific, non-selective
CC cation channel that mediates the transport of Na(+), K(+) and Cs(+)
CC ions equally well. Activated directly by increases in intracellular
CC Ca(2+), but is impermeable to it. Gating is voltage-dependent and
CC displays rapid activation and deactivation kinetics upon channel
CC stimulation even during sustained elevations in Ca(2+). Also activated
CC by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-
CC triphosphate-producing receptor agonists. The channel is blocked by
CC extracellular acidification. External acidification has 2 effects, a
CC fast reversible block of the current and a slower irreversible
CC enhancement of current inactivation. Is a highly temperature-sensitive,
CC heat activated channel showing a steep increase of inward currents at
CC temperatures between 15 and 35 degrees Celsius. Heat activation is due
CC to a shift of the voltage-dependent activation curve to negative
CC potentials. Activated by arachidonic acid in vitro. May be involved in
CC perception of bitter, sweet and umami tastes. May also be involved in
CC sensing semiochemicals. {ECO:0000269|PubMed:14634208}.
CC -!- INTERACTION:
CC Q9NZQ8; Q9UM19: HPCAL4; NbExp=3; IntAct=EBI-18161658, EBI-744820;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000250|UniProtKB:Q8TD43, ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1 {ECO:0000269|PubMed:10607831, ECO:0000269|PubMed:15489334,
CC ECO:0000269|PubMed:16554811};
CC IsoId=Q9NZQ8-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|Ref.4};
CC IsoId=Q9NZQ8-2; Sequence=VSP_052741;
CC Name=3 {ECO:0000269|PubMed:10607831};
CC IsoId=Q9NZQ8-3; Sequence=VSP_052742, VSP_052743;
CC -!- TISSUE SPECIFICITY: Strongly expressed in fetal brain, liver and
CC kidney, and in adult prostate, testis, ovary, colon and peripheral
CC blood leukocytes. Also expressed in a large proportion of Wilms' tumors
CC and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive
CC paternal expression. {ECO:0000269|PubMed:10607831}.
CC -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. LTrpC
CC subfamily. TRPM5 sub-subfamily. {ECO:0000305}.
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DR EMBL; AF177473; AAF26288.1; -; mRNA.
DR EMBL; AC124057; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC093787; AAH93787.1; -; mRNA.
DR EMBL; BC093789; AAH93789.1; -; mRNA.
DR EMBL; AJ270996; CAB66342.1; -; mRNA.
DR CCDS; CCDS31340.1; -. [Q9NZQ8-1]
DR RefSeq; NP_055370.1; NM_014555.3. [Q9NZQ8-1]
DR AlphaFoldDB; Q9NZQ8; -.
DR SMR; Q9NZQ8; -.
DR BioGRID; 118932; 2.
DR IntAct; Q9NZQ8; 2.
DR STRING; 9606.ENSP00000155858; -.
DR BindingDB; Q9NZQ8; -.
DR ChEMBL; CHEMBL1628468; -.
DR DrugCentral; Q9NZQ8; -.
DR GuidetoPHARMACOLOGY; 497; -.
DR TCDB; 1.A.4.5.3; the transient receptor potential ca(2+) channel (trp-cc) family.
DR iPTMnet; Q9NZQ8; -.
DR PhosphoSitePlus; Q9NZQ8; -.
DR BioMuta; TRPM5; -.
DR DMDM; 74753086; -.
DR PaxDb; Q9NZQ8; -.
DR PeptideAtlas; Q9NZQ8; -.
DR PRIDE; Q9NZQ8; -.
DR ProteomicsDB; 83486; -. [Q9NZQ8-1]
DR ProteomicsDB; 83487; -. [Q9NZQ8-2]
DR ProteomicsDB; 83488; -. [Q9NZQ8-3]
DR TopDownProteomics; Q9NZQ8-3; -. [Q9NZQ8-3]
DR Antibodypedia; 23152; 165 antibodies from 30 providers.
DR DNASU; 29850; -.
DR Ensembl; ENST00000155858.10; ENSP00000155858.5; ENSG00000070985.13. [Q9NZQ8-1]
DR GeneID; 29850; -.
DR KEGG; hsa:29850; -.
DR UCSC; uc001lwm.5; human. [Q9NZQ8-1]
DR CTD; 29850; -.
DR DisGeNET; 29850; -.
DR GeneCards; TRPM5; -.
DR HGNC; HGNC:14323; TRPM5.
DR HPA; ENSG00000070985; Tissue enhanced (epididymis, intestine, pancreas, seminal vesicle).
DR MIM; 604600; gene.
DR neXtProt; NX_Q9NZQ8; -.
DR OpenTargets; ENSG00000070985; -.
DR PharmGKB; PA37869; -.
DR VEuPathDB; HostDB:ENSG00000070985; -.
DR eggNOG; KOG3614; Eukaryota.
DR GeneTree; ENSGT00940000160588; -.
DR InParanoid; Q9NZQ8; -.
DR OMA; ICYCTAV; -.
DR OrthoDB; 738147at2759; -.
DR PhylomeDB; Q9NZQ8; -.
DR TreeFam; TF314204; -.
DR PathwayCommons; Q9NZQ8; -.
DR Reactome; R-HSA-3295583; TRP channels.
DR Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR SignaLink; Q9NZQ8; -.
DR BioGRID-ORCS; 29850; 12 hits in 1068 CRISPR screens.
DR GeneWiki; TRPM5; -.
DR GenomeRNAi; 29850; -.
DR Pharos; Q9NZQ8; Tchem.
DR PRO; PR:Q9NZQ8; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9NZQ8; protein.
DR Bgee; ENSG00000070985; Expressed in mucosa of transverse colon and 34 other tissues.
DR ExpressionAtlas; Q9NZQ8; baseline and differential.
DR Genevisible; Q9NZQ8; HS.
DR GO; GO:0030425; C:dendrite; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; TAS:ProtInc.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0005227; F:calcium activated cation channel activity; IBA:GO_Central.
DR GO; GO:0005261; F:cation channel activity; IBA:GO_Central.
DR GO; GO:0005216; F:ion channel activity; TAS:ProtInc.
DR GO; GO:0099604; F:ligand-gated calcium channel activity; IBA:GO_Central.
DR GO; GO:0005267; F:potassium channel activity; IEA:Ensembl.
DR GO; GO:0005272; F:sodium channel activity; TAS:Reactome.
DR GO; GO:0005244; F:voltage-gated ion channel activity; IEA:UniProtKB-KW.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0098655; P:cation transmembrane transport; IBA:GO_Central.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR GO; GO:0050909; P:sensory perception of taste; IEA:InterPro.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR029577; TRPM5.
DR InterPro; IPR041491; TRPM_SLOG.
DR PANTHER; PTHR13800:SF5; PTHR13800:SF5; 1.
DR Pfam; PF00520; Ion_trans; 1.
DR Pfam; PF18139; LSDAT_euk; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Coiled coil; Ion channel;
KW Ion transport; Ligand-gated ion channel; Membrane; Receptor;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport;
KW Voltage-gated channel.
FT CHAIN 1..1165
FT /note="Transient receptor potential cation channel
FT subfamily M member 5"
FT /id="PRO_0000328933"
FT TOPO_DOM 1..646
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 647..667
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 668..733
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 734..754
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 755..811
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 812..832
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 833..835
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 836..856
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 857..872
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 873..893
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 894..954
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 955..975
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 976..1165
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 481..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1126..1165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 553..581
FT /evidence="ECO:0000255"
FT COMPBIAS 483..499
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 155
FT /note="Q -> QVH (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_052741"
FT VAR_SEQ 870..872
FT /note="MKD -> KPV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10607831"
FT /id="VSP_052742"
FT VAR_SEQ 873..1165
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10607831"
FT /id="VSP_052743"
FT VARIANT 235
FT /note="N -> S (in dbSNP:rs886277)"
FT /id="VAR_052377"
FT VARIANT 254
FT /note="V -> A (in dbSNP:rs3986599)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_042578"
FT VARIANT 335
FT /note="V -> L (in dbSNP:rs34350821)"
FT /id="VAR_052378"
FT VARIANT 456
FT /note="A -> T (in dbSNP:rs34551253)"
FT /id="VAR_052379"
FT VARIANT 578
FT /note="R -> Q (in dbSNP:rs4929982)"
FT /id="VAR_059836"
SQ SEQUENCE 1165 AA; 131451 MW; C4AD5BAA866BE73B CRC64;
MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW
HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV
RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL
SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI
SRAVEQAAPW LILVGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT
KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR
VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR
SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG
FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM
GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV
RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV
YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL
LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL
EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM
DFMVFTLRLI HIFAIHKQLG PKIIVVERMM KDVFFFLFFL SVWLVAYGVT TQALLHPHDG
RLEWIFRRVL YRPYLQIFGQ IPLDEIDEAR VNCSTHPLLL EDSPSCPSLY ANWLVILLLV
TFLLVTNVLL MNLLIAMFSY TFQVVQGNAD MFWKFQRYNL IVEYHERPAL APPFILLSHL
SLTLRRVFKK EAEHKREHLE RDLPDPLDQK VVTWETVQKE NFLSKMEKRR RDSEGEVLRK
TAHRVDFIAK YLGGLREQEK RIKCLESQIN YCSVLVSSVA DVLAQGGGPR SSQHCGEGSQ
LVAADHRGGL DGWEQPGAGQ PPSDT
