ID   TRPR_ECO57              Reviewed;         108 AA.
AC   P0A882; P03032;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Trp operon repressor;
GN   Name=trpR; OrderedLocusNames=Z5995, ECs5351;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: This protein is an aporepressor. When complexed with L-
CC       tryptophan it binds the operator region of the trp operon (5'-
CC       ACTAGT-'3') and prevents the initiation of transcription. The complex
CC       also regulates trp repressor biosynthesis by binding to its regulatory
CC       region (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TrpR family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG59573.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38774.1; -; Genomic_DNA.
DR   PIR; A86139; A86139.
DR   PIR; G91297; G91297.
DR   RefSeq; NP_313378.1; NC_002695.1.
DR   RefSeq; WP_000068679.1; NZ_SWKA01000005.1.
DR   PDB; 2XDI; NMR; -; A/B=2-108.
DR   PDBsum; 2XDI; -.
DR   AlphaFoldDB; P0A882; -.
DR   SMR; P0A882; -.
DR   STRING; 155864.EDL933_5737; -.
DR   EnsemblBacteria; AAG59573; AAG59573; Z5995.
DR   EnsemblBacteria; BAB38774; BAB38774; ECs_5351.
DR   GeneID; 66671719; -.
DR   GeneID; 913491; -.
DR   KEGG; ece:Z5995; -.
DR   KEGG; ecs:ECs_5351; -.
DR   PATRIC; fig|386585.9.peg.5599; -.
DR   eggNOG; COG2973; Bacteria.
DR   HOGENOM; CLU_147939_0_0_6; -.
DR   OMA; GQMSQRE; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   DisProt; DP00810; -.
DR   Gene3D; 1.10.1270.10; -; 1.
DR   HAMAP; MF_00475; Trp_repressor; 1.
DR   InterPro; IPR000831; Trp_repress.
DR   InterPro; IPR013335; Trp_repress_bac.
DR   InterPro; IPR010921; Trp_repressor/repl_initiator.
DR   InterPro; IPR038116; TrpR-like_sf.
DR   PANTHER; PTHR38025; PTHR38025; 1.
DR   Pfam; PF01371; Trp_repressor; 1.
DR   PIRSF; PIRSF003196; Trp_repressor; 1.
DR   SUPFAM; SSF48295; SSF48295; 1.
DR   TIGRFAMs; TIGR01321; TrpR; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..108
FT                   /note="Trp operon repressor"
FT                   /id="PRO_0000196495"
FT   DNA_BIND        68..91
FT                   /evidence="ECO:0000250"
FT   STRAND          13..16
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   HELIX           17..32
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   HELIX           36..42
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   HELIX           45..63
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   TURN            68..70
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   STRAND          75..81
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   TURN            89..91
FT                   /evidence="ECO:0007829|PDB:2XDI"
FT   HELIX           93..104
FT                   /evidence="ECO:0007829|PDB:2XDI"
SQ   SEQUENCE   108 AA;  12355 MW;  FDFF8A60EC4FE7BE CRC64;
     MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA LGTRVRIVEE
     LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL EEVLLKSD