TRS20_YEAST
ID TRS20_YEAST Reviewed; 175 AA.
AC P38334; D6VQQ0;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Trafficking protein particle complex subunit 20;
DE Short=TRAPP subunit 20;
DE AltName: Full=Transport protein particle 20 kDa subunit;
GN Name=TRS20; OrderedLocusNames=YBR254C; ORFNames=YBR1722;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8465606; DOI=10.1002/yea.320090210;
RA Doignon F., Biteau N., Crouzet M., Aigle M.;
RT "The complete sequence of a 19,482 bp segment located on the right arm of
RT chromosome II from Saccharomyces cerevisiae.";
RL Yeast 9:189-199(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND IDENTIFICATION IN THE TRAPP II COMPLEX.
RX PubMed=9564032; DOI=10.1093/emboj/17.9.2494;
RA Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L.,
RA Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.;
RT "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates
RT vesicle docking and fusion.";
RL EMBO J. 17:2494-2503(1998).
RN [5]
RP FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX,
RP FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, AND
RP SUBCELLULAR LOCATION.
RX PubMed=11239471; DOI=10.1016/s1097-2765(01)00190-3;
RA Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M.,
RA Ferro-Novick S.;
RT "TRAPP I implicated in the specificity of tethering in ER-to-Golgi
RT transport.";
RL Mol. Cell 7:433-442(2001).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION IN THE TRAP II COMPLEX, AND FUNCTION OF THE TRAP II COMPLEX.
RX PubMed=20972447; DOI=10.1038/nsmb.1914;
RA Yip C.K., Berscheminski J., Walz T.;
RT "Molecular architecture of the TRAPPII complex and implications for vesicle
RT tethering.";
RL Nat. Struct. Mol. Biol. 17:1298-1304(2010).
RN [8]
RP IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, AND FUNCTION
RP OF THE TRAPP III COMPLEX.
RX PubMed=20375281; DOI=10.1073/pnas.1000063107;
RA Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R.,
RA Brumell J.H., Ferro-Novick S., Klionsky D.J.;
RT "Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote
RT autophagy.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010).
CC -!- FUNCTION: Component of the TRAPP I, TRAPP II and TRAPP III complexes
CC which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP
CC I plays a key role in the late stages of endoplasmic reticulum to Golgi
CC traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III
CC plays a role in autophagosome formation. {ECO:0000269|PubMed:11239471,
CC ECO:0000269|PubMed:20375281, ECO:0000269|PubMed:20972447,
CC ECO:0000269|PubMed:9564032}.
CC -!- SUBUNIT: Part of the multisubunit TRAPP (transport protein particle) I
CC complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of
CC the multisubunit TRAPP (transport protein particle) II complex composed
CC of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and
CC TRS130. Part of the multisubunit TRAPP (transport protein particle) III
CC complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.
CC {ECO:0000269|PubMed:11239471, ECO:0000269|PubMed:20375281,
CC ECO:0000269|PubMed:20972447, ECO:0000269|PubMed:9564032}.
CC -!- INTERACTION:
CC P38334; P36149: BET3; NbExp=11; IntAct=EBI-19468, EBI-3567;
CC P38334; Q03660: TRS130; NbExp=2; IntAct=EBI-19468, EBI-19461;
CC P38334; Q03337: TRS31; NbExp=4; IntAct=EBI-19468, EBI-38770;
CC P38334; P46944: TRS85; NbExp=6; IntAct=EBI-19468, EBI-19492;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network. Endoplasmic
CC reticulum. Preautophagosomal structure.
CC -!- MISCELLANEOUS: Present with 1200 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TRAPP small subunits family. Sedlin
CC subfamily. {ECO:0000305}.
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DR EMBL; X70529; CAA49918.1; -; Genomic_DNA.
DR EMBL; Z36123; CAA85217.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07370.1; -; Genomic_DNA.
DR PIR; S32957; S32957.
DR RefSeq; NP_009813.1; NM_001178602.1.
DR PDB; 7E2C; EM; 4.18 A; H=1-175.
DR PDB; 7E2D; EM; 3.71 A; H=1-175.
DR PDB; 7E8S; EM; 4.36 A; H/S=1-175.
DR PDB; 7E8T; EM; 3.80 A; H=1-175.
DR PDB; 7E93; EM; 6.54 A; H/S=1-175.
DR PDB; 7E94; EM; 4.67 A; H/S=1-175.
DR PDB; 7EA3; EM; 4.31 A; H/U=1-175.
DR PDB; 7KMT; EM; 3.70 A; K=1-175.
DR PDBsum; 7E2C; -.
DR PDBsum; 7E2D; -.
DR PDBsum; 7E8S; -.
DR PDBsum; 7E8T; -.
DR PDBsum; 7E93; -.
DR PDBsum; 7E94; -.
DR PDBsum; 7EA3; -.
DR PDBsum; 7KMT; -.
DR AlphaFoldDB; P38334; -.
DR SMR; P38334; -.
DR BioGRID; 32949; 556.
DR ComplexPortal; CPX-1383; TRAPPIII protein complex.
DR ComplexPortal; CPX-1939; TRAPPII protein complex.
DR ComplexPortal; CPX-1940; TRAPPI protein complex.
DR DIP; DIP-1711N; -.
DR IntAct; P38334; 22.
DR MINT; P38334; -.
DR STRING; 4932.YBR254C; -.
DR MaxQB; P38334; -.
DR PaxDb; P38334; -.
DR PRIDE; P38334; -.
DR EnsemblFungi; YBR254C_mRNA; YBR254C; YBR254C.
DR GeneID; 852556; -.
DR KEGG; sce:YBR254C; -.
DR SGD; S000000458; TRS20.
DR VEuPathDB; FungiDB:YBR254C; -.
DR eggNOG; KOG3487; Eukaryota.
DR HOGENOM; CLU_085828_0_0_1; -.
DR InParanoid; P38334; -.
DR OMA; VDKFNQW; -.
DR BioCyc; YEAST:G3O-29179-MON; -.
DR Reactome; R-SCE-204005; COPII-mediated vesicle transport.
DR Reactome; R-SCE-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR PRO; PR:P38334; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38334; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000407; C:phagophore assembly site; IC:ComplexPortal.
DR GO; GO:0030008; C:TRAPP complex; IBA:GO_Central.
DR GO; GO:1990070; C:TRAPPI protein complex; IDA:SGD.
DR GO; GO:1990071; C:TRAPPII protein complex; IDA:SGD.
DR GO; GO:1990072; C:TRAPPIII protein complex; IDA:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IDA:ComplexPortal.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IC:ComplexPortal.
DR GO; GO:0016236; P:macroautophagy; IC:ComplexPortal.
DR GO; GO:0065003; P:protein-containing complex assembly; IMP:SGD.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IC:ComplexPortal.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR006722; Sedlin.
DR PANTHER; PTHR12403; PTHR12403; 1.
DR Pfam; PF04628; Sedlin_N; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Autophagy; Endoplasmic reticulum; ER-Golgi transport;
KW Golgi apparatus; Reference proteome; Transport.
FT CHAIN 1..175
FT /note="Trafficking protein particle complex subunit 20"
FT /id="PRO_0000211568"
SQ SEQUENCE 175 AA; 19700 MW; 82285CAEAC2735D9 CRC64;
MPQYFAIIGK KDNPVYEIEF TNAENPQGFP QDLKELNPFI LHASLDIVED LQWQINPTSQ
LNGNGGNGSN GGGGFLRSRA VNNTDNCYLG KVDHFYGLAI TAYISYSGMK FVMIHGNSAN
SSVVIDDNNM RSFYQEVHEL YVKTLMNPFY KITDPIRSPA FDSRVRTLAR KHLSK
