TRS23_YEAST
ID TRS23_YEAST Reviewed; 219 AA.
AC Q03784; D6VSM6;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Trafficking protein particle complex subunit 23;
DE Short=TRAPP subunit 23;
DE AltName: Full=Transport protein particle 23 kDa subunit;
GN Name=TRS23; OrderedLocusNames=YDR246W; ORFNames=YD8419.13;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION IN THE TRAPP II COMPLEX.
RX PubMed=9564032; DOI=10.1093/emboj/17.9.2494;
RA Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L.,
RA Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.;
RT "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates
RT vesicle docking and fusion.";
RL EMBO J. 17:2494-2503(1998).
RN [4]
RP FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX,
RP AND SUBCELLULAR LOCATION.
RX PubMed=11239471; DOI=10.1016/s1097-2765(01)00190-3;
RA Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M.,
RA Ferro-Novick S.;
RT "TRAPP I implicated in the specificity of tethering in ER-to-Golgi
RT transport.";
RL Mol. Cell 7:433-442(2001).
RN [5]
RP IDENTIFICATION IN TRAPP COMPLEX.
RX PubMed=11805826; DOI=10.1038/415141a;
RA Gavin A.-C., Boesche M., Krause R., Grandi P., Marzioch M., Bauer A.,
RA Schultz J., Rick J.M., Michon A.-M., Cruciat C.-M., Remor M., Hoefert C.,
RA Schelder M., Brajenovic M., Ruffner H., Merino A., Klein K., Hudak M.,
RA Dickson D., Rudi T., Gnau V., Bauch A., Bastuck S., Huhse B., Leutwein C.,
RA Heurtier M.-A., Copley R.R., Edelmann A., Querfurth E., Rybin V.,
RA Drewes G., Raida M., Bouwmeester T., Bork P., Seraphin B., Kuster B.,
RA Neubauer G., Superti-Furga G.;
RT "Functional organization of the yeast proteome by systematic analysis of
RT protein complexes.";
RL Nature 415:141-147(2002).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP IDENTIFICATION IN THE TRAP II COMPLEX, AND FUNCTION OF THE TRAP II COMPLEX.
RX PubMed=20972447; DOI=10.1038/nsmb.1914;
RA Yip C.K., Berscheminski J., Walz T.;
RT "Molecular architecture of the TRAPPII complex and implications for vesicle
RT tethering.";
RL Nat. Struct. Mol. Biol. 17:1298-1304(2010).
RN [8]
RP IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, AND FUNCTION
RP OF THE TRAPP III COMPLEX.
RX PubMed=20375281; DOI=10.1073/pnas.1000063107;
RA Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R.,
RA Brumell J.H., Ferro-Novick S., Klionsky D.J.;
RT "Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote
RT autophagy.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010).
CC -!- FUNCTION: Component of the TRAPP I, TRAPP II and TRAPP III complexes
CC which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP
CC I plays a key role in the late stages of endoplasmic reticulum to Golgi
CC traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III
CC plays a role in autophagosome formation. {ECO:0000269|PubMed:11239471,
CC ECO:0000269|PubMed:20375281, ECO:0000269|PubMed:20972447}.
CC -!- SUBUNIT: Part of the multisubunit TRAPP (transport protein particle) I
CC complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of
CC the multisubunit TRAPP (transport protein particle) II complex composed
CC of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and
CC TRS130. Part of the multisubunit TRAPP (transport protein particle) III
CC complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.
CC {ECO:0000269|PubMed:11239471, ECO:0000269|PubMed:11805826,
CC ECO:0000269|PubMed:20375281, ECO:0000269|PubMed:20972447,
CC ECO:0000269|PubMed:9564032}.
CC -!- INTERACTION:
CC Q03784; P36149: BET3; NbExp=11; IntAct=EBI-19474, EBI-3567;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network. Endoplasmic
CC reticulum. Preautophagosomal structure.
CC -!- MISCELLANEOUS: Present with 3130 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TRAPP small subunits family. TRAPPC4
CC subfamily. {ECO:0000305}.
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DR EMBL; Z49701; CAA89732.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12086.1; -; Genomic_DNA.
DR PIR; S54542; S54542.
DR RefSeq; NP_010532.3; NM_001180554.3.
DR PDB; 3CUE; X-ray; 3.70 A; A/G/M/S=1-219.
DR PDB; 7E2C; EM; 4.18 A; E=1-219.
DR PDB; 7E2D; EM; 3.71 A; E=1-219.
DR PDB; 7E8S; EM; 4.36 A; E/P=1-219.
DR PDB; 7E8T; EM; 3.80 A; E=1-219.
DR PDB; 7E93; EM; 6.54 A; E/P=1-219.
DR PDB; 7E94; EM; 4.67 A; E/P=1-219.
DR PDB; 7EA3; EM; 4.31 A; E/R=1-219.
DR PDB; 7KMT; EM; 3.70 A; H=1-219.
DR PDBsum; 3CUE; -.
DR PDBsum; 7E2C; -.
DR PDBsum; 7E2D; -.
DR PDBsum; 7E8S; -.
DR PDBsum; 7E8T; -.
DR PDBsum; 7E93; -.
DR PDBsum; 7E94; -.
DR PDBsum; 7EA3; -.
DR PDBsum; 7KMT; -.
DR AlphaFoldDB; Q03784; -.
DR SMR; Q03784; -.
DR BioGRID; 32297; 553.
DR ComplexPortal; CPX-1383; TRAPPIII protein complex.
DR ComplexPortal; CPX-1939; TRAPPII protein complex.
DR ComplexPortal; CPX-1940; TRAPPI protein complex.
DR DIP; DIP-5813N; -.
DR IntAct; Q03784; 5.
DR MINT; Q03784; -.
DR STRING; 4932.YDR246W; -.
DR MaxQB; Q03784; -.
DR PaxDb; Q03784; -.
DR PRIDE; Q03784; -.
DR EnsemblFungi; YDR246W_mRNA; YDR246W; YDR246W.
DR GeneID; 851833; -.
DR KEGG; sce:YDR246W; -.
DR SGD; S000002654; TRS23.
DR VEuPathDB; FungiDB:YDR246W; -.
DR eggNOG; KOG3369; Eukaryota.
DR GeneTree; ENSGT00940000153761; -.
DR HOGENOM; CLU_053380_0_0_1; -.
DR InParanoid; Q03784; -.
DR OMA; YSMEMPI; -.
DR BioCyc; YEAST:G3O-29819-MON; -.
DR Reactome; R-SCE-204005; COPII-mediated vesicle transport.
DR Reactome; R-SCE-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR EvolutionaryTrace; Q03784; -.
DR PRO; PR:Q03784; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03784; protein.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0000407; C:phagophore assembly site; IC:ComplexPortal.
DR GO; GO:0030008; C:TRAPP complex; IBA:GO_Central.
DR GO; GO:1990070; C:TRAPPI protein complex; IDA:SGD.
DR GO; GO:1990071; C:TRAPPII protein complex; IDA:SGD.
DR GO; GO:1990072; C:TRAPPIII protein complex; IDA:SGD.
DR GO; GO:0051276; P:chromosome organization; IMP:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IDA:ComplexPortal.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IC:ComplexPortal.
DR GO; GO:0016236; P:macroautophagy; IC:ComplexPortal.
DR GO; GO:0050790; P:regulation of catalytic activity; IEA:GOC.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IC:ComplexPortal.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR007233; TRAPPC.
DR PANTHER; PTHR23249; PTHR23249; 1.
DR Pfam; PF04099; Sybindin; 3.
DR SMART; SM01399; Sybindin; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Autophagy; Endoplasmic reticulum; ER-Golgi transport;
KW Golgi apparatus; Reference proteome; Transport.
FT CHAIN 1..219
FT /note="Trafficking protein particle complex subunit 23"
FT /id="PRO_0000211571"
SQ SEQUENCE 219 AA; 24863 MW; 8B32B1D5FE3846ED CRC64;
MAIETILVIN KSGGLIYQRN FTNDEQKLNS NEYLILASTL HGVFAIASQL TPKALQLTQQ
TNIENTIPYI PYVGMSSNRS DTRNGGGNNN KHTNNEKLGS FKGDDFFKEP FTNWNKSGLR
QLCTDQFTMF IYQTLTGLKF VAISSSVMPQ RQPTIATTDK PDRPKSTSNL AIQIADNFLR
KVYCLYSDYV MKDPSYSMEM PIRSNLFDEK VKKMVENLQ
