TRSF_MUSDO
ID TRSF_MUSDO Reviewed; 367 AA.
AC A0A1I8MUL8; A0A1I8MUL9; D0VEM0;
DT 30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT 18-JAN-2017, sequence version 1.
DT 25-MAY-2022, entry version 19.
DE RecName: Full=Female-specific protein transformer {ECO:0000303|PubMed:19841093};
DE Short=Md-tra {ECO:0000303|PubMed:19841093};
GN Name=tra {ECO:0000303|PubMed:19841093};
OS Musca domestica (House fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC Muscidae; Musca.
OX NCBI_TaxID=7370;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND ALTERNATIVE SPLICING.
RX PubMed=19841093; DOI=10.1534/genetics.109.109249;
RA Hediger M., Henggeler C., Meier N., Perez R., Saccone G., Bopp D.;
RT "Molecular characterization of the key switch F provides a basis for
RT understanding the rapid divergence of the sex-determining pathway in the
RT housefly.";
RL Genetics 184:155-170(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=aabys;
RX PubMed=25315136; DOI=10.1186/s13059-014-0466-3;
RA Scott J.G., Warren W.C., Beukeboom L.W., Bopp D., Clark A.G., Giers S.D.,
RA Hediger M., Jones A.K., Kasai S., Leichter C.A., Li M., Meisel R.P.,
RA Minx P., Murphy T.D., Nelson D.R., Reid W.R., Rinkevich F.D.,
RA Robertson H.M., Sackton T.B., Sattelle D.B., Thibaud-Nissen F.,
RA Tomlinson C., van de Zande L., Walden K.K., Wilson R.K., Liu N.;
RT "Genome of the house fly, Musca domestica L., a global vector of diseases
RT with adaptations to a septic environment.";
RL Genome Biol. 15:RESEARCH0466.1-RESEARCH0466.16(2014).
RN [3]
RP FUNCTION.
RX PubMed=28495751; DOI=10.1126/science.aam5498;
RA Sharma A., Heinze S.D., Wu Y., Kohlbrenner T., Morilla I., Brunner C.,
RA Wimmer E.A., van de Zande L., Robinson M.D., Beukeboom L.W., Bopp D.;
RT "Male sex in houseflies is determined by Mdmd, a paralog of the generic
RT splice factor gene CWC22.";
RL Science 356:642-645(2017).
CC -!- FUNCTION: Sex differentiation protein controlling female somatic sexual
CC differentiation (PubMed:19841093, PubMed:28495751). May act by
CC promoting the formation of a splicing enhancer complex (By similarity).
CC {ECO:0000250|UniProtKB:P11596, ECO:0000269|PubMed:19841093,
CC ECO:0000269|PubMed:28495751}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=The sexual regulation of tra occurs through a mechanism of
CC sex-specific alternative mRNA splicing. The active state of tra is
CC initially established by maternally provided tra (PubMed:19841093).
CC Once activated, zygotic tra perpetuates its female-promoting function
CC by a positive splicing feedback loop throughout development
CC (PubMed:19841093). The splice forms expressed in males is not
CC translated: splice form expressed in males is regulated by the male
CC determiner protein Mdmd (PubMed:28495751).
CC {ECO:0000269|PubMed:19841093, ECO:0000269|PubMed:28495751};
CC Name=1;
CC IsoId=A0A1I8MUL8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0A1I8MUL8-2; Sequence=VSP_059060;
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DR EMBL; GU070694; ACY40709.1; -; Genomic_DNA.
DR RefSeq; XP_005190756.1; XM_005190699.3. [A0A1I8MUL8-1]
DR RefSeq; XP_011295491.1; XM_011297189.2. [A0A1I8MUL8-2]
DR RefSeq; XP_011295493.1; XM_011297191.2. [A0A1I8MUL8-2]
DR RefSeq; XP_019894915.1; XM_020039356.1. [A0A1I8MUL8-2]
DR AlphaFoldDB; A0A1I8MUL8; -.
DR GeneID; 101888218; -.
DR KEGG; mde:101888218; -.
DR VEuPathDB; VectorBase:MDOA008597; -.
DR eggNOG; ENOG502TD84; Eukaryota.
DR Proteomes; UP000095301; Unplaced.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0030237; P:female sex determination; IDA:UniProtKB.
DR GO; GO:0046660; P:female sex differentiation; IDA:UniProtKB.
PE 3: Inferred from homology;
KW Alternative splicing; Differentiation; Sexual differentiation.
FT CHAIN 1..367
FT /note="Female-specific protein transformer"
FT /id="PRO_0000441317"
FT REGION 86..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..156
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 164..184
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..221
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..236
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 237..253
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..64
FT /note="MEQTSMGKDSSKTAIAKFVPDDDGVLKHTIKVHQQPTSATEKKGPSMIARSN
FT DLIEEIQIKRRF -> MNLYIIYLPTNDNN (in isoform 2)"
FT /id="VSP_059060"
FT CONFLICT 282
FT /note="Q -> P (in Ref. 1; ACY40709)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 367 AA; 42295 MW; DCA501DEAD81FE38 CRC64;
MEQTSMGKDS SKTAIAKFVP DDDGVLKHTI KVHQQPTSAT EKKGPSMIAR SNDLIEEIQI
KRRFGEGTKP LFERDDVKVN TVADGESISS KKIKSHKASS PSKGVNGLVK QNSPDVTQKF
TKKYGSSENP DFRRHSSYEK DNYHKSNSKS GVHLEGHEGN YRPYFSDPIR ERDRLRRLYG
SPNERRSRSH SPSSNRRRSS HRSRRGSGSP RSRRYTSRHR RRSNSQDRTS WKHNPEHRTS
RRSRTRSPRG NRSRRRSSTS SNEDDEREYR HRHHRSQERS YQNVLPTLPP ALTNYPCHYH
VAPMLALPGV QHRPFLPMVA SVRHLPPQAL YGGLAGAMPF IPMPMTAYRP HLGHRYPPPR
HKINKKN
