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TRT14_ASPTN
ID   TRT14_ASPTN             Reviewed;         142 AA.
AC   Q0C8A2;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Isomerase trt14 {ECO:0000303|PubMed:25671343};
DE            EC=5.-.-.- {ECO:0000269|PubMed:25671343, ECO:0000269|PubMed:28759016};
DE   AltName: Full=Terretonin synthesis protein 14 {ECO:0000303|PubMed:23116177};
GN   Name=trt14 {ECO:0000303|PubMed:23116177}; ORFNames=ATEG_10082;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=22549923; DOI=10.1002/cbic.201200124;
RA   Itoh T., Tokunaga K., Radhakrishnan E.K., Fujii I., Abe I., Ebizuka Y.,
RA   Kushiro T.;
RT   "Identification of a key prenyltransferase involved in biosynthesis of the
RT   most abundant fungal meroterpenoids derived from 3,5-dimethylorsellinic
RT   acid.";
RL   ChemBioChem 13:1132-1135(2012).
RN   [3]
RP   FUNCTION.
RX   PubMed=22782788; DOI=10.1002/cbic.201200369;
RA   Matsuda Y., Awakawa T., Itoh T., Wakimoto T., Kushiro T., Fujii I.,
RA   Ebizuka Y., Abe I.;
RT   "Terretonin biosynthesis requires methylation as essential step for
RT   cyclization.";
RL   ChemBioChem 13:1738-1741(2012).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23116177; DOI=10.1021/ol302682z;
RA   Guo C.J., Knox B.P., Chiang Y.M., Lo H.C., Sanchez J.F., Lee K.H.,
RA   Oakley B.R., Bruno K.S., Wang C.C.;
RT   "Molecular genetic characterization of a cluster in A. terreus for
RT   biosynthesis of the meroterpenoid terretonin.";
RL   Org. Lett. 14:5684-5687(2012).
RN   [5]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=25671343; DOI=10.1021/jacs.5b00570;
RA   Matsuda Y., Iwabuchi T., Wakimoto T., Awakawa T., Abe I.;
RT   "Uncovering the unusual D-ring construction in terretonin biosynthesis by
RT   collaboration of a multifunctional cytochrome P450 and a unique
RT   isomerase.";
RL   J. Am. Chem. Soc. 137:3393-3401(2015).
RN   [6] {ECO:0007744|PDB:5WQF, ECO:0007744|PDB:5WQG, ECO:0007744|PDB:5WQH, ECO:0007744|PDB:5WQI}
RP   X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, SUBUNIT,
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND
RP   MUTAGENESIS OF TYR-19; ARG-31; SER-37; ARG-39 AND ARG-48.
RX   PubMed=28759016; DOI=10.1038/nchembio.2443;
RA   Mori T., Iwabuchi T., Hoshino S., Wang H., Matsuda Y., Abe I.;
RT   "Molecular basis for the unusual ring reconstruction in fungal
RT   meroterpenoid biogenesis.";
RL   Nat. Chem. Biol. 13:1066-1073(2017).
CC   -!- FUNCTION: Isomerase; part of the gene cluster that mediates the
CC       biosynthesis of terretonin, a fungal meroterpenoid that acts as a
CC       mycotoxin (PubMed:22549923, PubMed:23116177, PubMed:25671343). The
CC       first step of the pathway is the synthesis of 3,5-dimethylorsellinic
CC       acid (DMOA) by the polyketide synthase trt4 (PubMed:22549923,
CC       PubMed:23116177). DMOA is then prenylated into farnesyl-DMOA by the
CC       polyprenyl transferase trt2 (PubMed:22549923, PubMed:22782788,
CC       PubMed:23116177). Methylation by the methyltransferase trt5 then leads
CC       to farnesyl-DMOA methyl ester which is further subject to epoxidation
CC       by the FAD-dependent monooxygenase trt8 to yield epoxyfarnesyl-DMOA
CC       methyl ester (PubMed:22549923, PubMed:22782788, PubMed:23116177).
CC       Cyclization of epoxyfarnesyl-DMOA methyl ester by the terpene cyclase
CC       trt1 leads to a tetracycle intermediate which is in turn converted to
CC       preterretonin (PubMed:22549923, PubMed:22782788, PubMed:23116177).
CC       Dehydrogenase trt9 comes next to transform preterretonin to
CC       preterrenoid (PubMed:22549923, PubMed:23116177). The FAD-dependent
CC       monooxygenase trt3 is then required for the C-hydroxylation at C16 of
CC       preterrenoid to yield terrenoid (PubMed:22549923, PubMed:23116177). The
CC       cytochrome P450 trt6 catalyzes three successive oxidations to transform
CC       terrenoid into an unstable intermediate, which then undergoes the D-
CC       ring expansion and unusual rearrangement of the methoxy group to afford
CC       the core skeleton of terretonin (PubMed:25671343, PubMed:28759016).
CC       Trt14 catalyzes the D-ring expansion of terretonin involving
CC       intramolecular methoxy rearrangement as well as the hydrolysis of the
CC       expanded D-ring and the methyl ester moiety (PubMed:25671343,
CC       PubMed:28759016). Finally, the nonheme iron-dependent dioxygenase trt7
CC       accomplishes the last two oxidation reactions steps to complete the
CC       biosynthesis of terretonin (PubMed:25671343). Terretonin C is produced
CC       via spontaneous decarboxylation of the terretonin precursor
CC       (PubMed:23116177). Another shunt product of the terretonin biosynthesis
CC       is dihydrofarnesyl-DMOA, derived from epoxyfarnesyl-DMOA through
CC       hydrolysis of the epoxide (PubMed:22549923, PubMed:22782788,
CC       PubMed:23116177). {ECO:0000269|PubMed:22549923,
CC       ECO:0000269|PubMed:22782788, ECO:0000269|PubMed:23116177,
CC       ECO:0000269|PubMed:25671343, ECO:0000269|PubMed:28759016}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=169.2 uM for the stable regioisomer of the product of trt6
CC         {ECO:0000269|PubMed:28759016};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:23116177, ECO:0000269|PubMed:28759016}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:28759016}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the synthesis of terretonin but
CC       accumulates terretonin C (PubMed:23116177).
CC       {ECO:0000269|PubMed:23116177}.
CC   -!- SIMILARITY: Belongs to the trt14 isomerase family. {ECO:0000305}.
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DR   EMBL; CH476609; EAU29531.1; -; Genomic_DNA.
DR   RefSeq; XP_001209384.1; XM_001209384.1.
DR   PDB; 5WQF; X-ray; 2.00 A; A/B/C/D/E/F=1-142.
DR   PDB; 5WQG; X-ray; 2.30 A; A/B/C/D/E/F=1-142.
DR   PDB; 5WQH; X-ray; 2.10 A; A/B/C/D/E/F=1-142.
DR   PDB; 5WQI; X-ray; 1.90 A; A/B/C/D/E/F=1-142.
DR   PDBsum; 5WQF; -.
DR   PDBsum; 5WQG; -.
DR   PDBsum; 5WQH; -.
DR   PDBsum; 5WQI; -.
DR   AlphaFoldDB; Q0C8A2; -.
DR   SMR; Q0C8A2; -.
DR   EnsemblFungi; EAU29531; EAU29531; ATEG_10082.
DR   GeneID; 4319489; -.
DR   VEuPathDB; FungiDB:ATEG_10082; -.
DR   eggNOG; ENOG502S4TQ; Eukaryota.
DR   HOGENOM; CLU_108113_3_0_1; -.
DR   OMA; NEYMIVL; -.
DR   OrthoDB; 1409451at2759; -.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   3D-structure; Isomerase; Reference proteome.
FT   CHAIN           1..142
FT                   /note="Isomerase trt14"
FT                   /id="PRO_0000436599"
FT   REGION          37..39
FT                   /note="Substrate"
FT                   /evidence="ECO:0007744|PDB:5WQG, ECO:0007744|PDB:5WQH,
FT                   ECO:0007744|PDB:5WQI"
FT   BINDING         19
FT                   /ligand="substrate"
FT                   /evidence="ECO:0007744|PDB:5WQH, ECO:0007744|PDB:5WQI"
FT   BINDING         39
FT                   /ligand="substrate"
FT                   /evidence="ECO:0007744|PDB:5WQI"
FT   BINDING         48
FT                   /ligand="substrate"
FT                   /evidence="ECO:0007744|PDB:5WQG, ECO:0007744|PDB:5WQH,
FT                   ECO:0007744|PDB:5WQI"
FT   MUTAGEN         19
FT                   /note="Y->A: Leads to a dramatic decrease of the catalytic
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:28759016"
FT   MUTAGEN         19
FT                   /note="Y->F: Alters the stereochemistry and the specificity
FT                   of the enzyme reaction."
FT                   /evidence="ECO:0000269|PubMed:28759016"
FT   MUTAGEN         31
FT                   /note="R->A: Does not significantly affect the catalytic
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:28759016"
FT   MUTAGEN         37
FT                   /note="S->V: Does not significantly affect the catalytic
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:28759016"
FT   MUTAGEN         39
FT                   /note="R->A: Leads to a dramatic decrease of the catalytic
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:28759016"
FT   MUTAGEN         48
FT                   /note="R->A: Leads to a dramatic decrease of the catalytic
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:28759016"
FT   HELIX           5..21
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   HELIX           24..27
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   TURN            28..30
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          31..39
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   HELIX           42..44
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   HELIX           50..61
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          64..72
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   HELIX           74..76
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          78..80
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   TURN            81..84
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          85..96
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          99..111
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   STRAND          115..125
FT                   /evidence="ECO:0007829|PDB:5WQI"
FT   HELIX           127..138
FT                   /evidence="ECO:0007829|PDB:5WQI"
SQ   SEQUENCE   142 AA;  16134 MW;  7598D4A2D31C9D1A CRC64;
     MSPTREDLVA TAKLFIAKYN EFTPESIISV RTPNSVSHRL FPTRNATRNI GESMEACANA
     KEVFKSLTVS VIDDNDTIVD ERTRKVVFYL ASRGDTIVGE WKSECIFIFQ MSEDGKLVDR
     IWAGFDTAYM DEFESRLDGI TF
 
 
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