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TRT9_ASPTN
ID   TRT9_ASPTN              Reviewed;         233 AA.
AC   Q0C898;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Short chain dehydrogenase trt9 {ECO:0000303|PubMed:22549923};
DE            EC=1.1.1.- {ECO:0000305|PubMed:23116177};
DE   AltName: Full=Terretonin synthesis protein 9 {ECO:0000303|PubMed:22549923};
GN   Name=trt9 {ECO:0000303|PubMed:22549923}; ORFNames=ATEG_10086;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RX   PubMed=22549923; DOI=10.1002/cbic.201200124;
RA   Itoh T., Tokunaga K., Radhakrishnan E.K., Fujii I., Abe I., Ebizuka Y.,
RA   Kushiro T.;
RT   "Identification of a key prenyltransferase involved in biosynthesis of the
RT   most abundant fungal meroterpenoids derived from 3,5-dimethylorsellinic
RT   acid.";
RL   ChemBioChem 13:1132-1135(2012).
RN   [3]
RP   FUNCTION.
RX   PubMed=22782788; DOI=10.1002/cbic.201200369;
RA   Matsuda Y., Awakawa T., Itoh T., Wakimoto T., Kushiro T., Fujii I.,
RA   Ebizuka Y., Abe I.;
RT   "Terretonin biosynthesis requires methylation as essential step for
RT   cyclization.";
RL   ChemBioChem 13:1738-1741(2012).
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=23116177; DOI=10.1021/ol302682z;
RA   Guo C.J., Knox B.P., Chiang Y.M., Lo H.C., Sanchez J.F., Lee K.H.,
RA   Oakley B.R., Bruno K.S., Wang C.C.;
RT   "Molecular genetic characterization of a cluster in A. terreus for
RT   biosynthesis of the meroterpenoid terretonin.";
RL   Org. Lett. 14:5684-5687(2012).
RN   [5]
RP   FUNCTION.
RX   PubMed=25671343; DOI=10.1021/jacs.5b00570;
RA   Matsuda Y., Iwabuchi T., Wakimoto T., Awakawa T., Abe I.;
RT   "Uncovering the unusual D-ring construction in terretonin biosynthesis by
RT   collaboration of a multifunctional cytochrome P450 and a unique
RT   isomerase.";
RL   J. Am. Chem. Soc. 137:3393-3401(2015).
RN   [6]
RP   FUNCTION.
RX   PubMed=28759016; DOI=10.1038/nchembio.2443;
RA   Mori T., Iwabuchi T., Hoshino S., Wang H., Matsuda Y., Abe I.;
RT   "Molecular basis for the unusual ring reconstruction in fungal
RT   meroterpenoid biogenesis.";
RL   Nat. Chem. Biol. 13:1066-1073(2017).
CC   -!- FUNCTION: Short chain dehydrogenase; part of the gene cluster that
CC       mediates the biosynthesis of terretonin, a fungal meroterpenoid that
CC       acts as a mycotoxin (PubMed:22549923, PubMed:23116177,
CC       PubMed:25671343). The first step of the pathway is the synthesis of
CC       3,5-dimethylorsellinic acid (DMOA) by the polyketide synthase trt4
CC       (PubMed:22549923, PubMed:23116177). DMOA is then prenylated into
CC       farnesyl-DMOA by the polyprenyl transferase trt2 (PubMed:22549923,
CC       PubMed:22782788, PubMed:23116177). Methylation by the methyltransferase
CC       trt5 then leads to farnesyl-DMOA methyl ester which is further subject
CC       to epoxidation by the FAD-dependent monooxygenase trt8 to yield
CC       epoxyfarnesyl-DMOA methyl ester (PubMed:22549923, PubMed:22782788,
CC       PubMed:23116177). Cyclization of epoxyfarnesyl-DMOA methyl ester by the
CC       terpene cyclase trt1 leads to a tetracycle intermediate which is in
CC       turn converted to preterretonin (PubMed:22549923, PubMed:22782788,
CC       PubMed:23116177). Dehydrogenase trt9 comes next to transform
CC       preterretonin to preterrenoid (PubMed:22549923, PubMed:23116177). The
CC       FAD-dependent monooxygenase trt3 is then required for the C-
CC       hydroxylation at C16 of preterrenoid to yield terrenoid
CC       (PubMed:22549923, PubMed:23116177). The cytochrome P450 trt6 catalyzes
CC       three successive oxidations to transform terrenoid into an unstable
CC       intermediate, which then undergoes the D-ring expansion and unusual
CC       rearrangement of the methoxy group to afford the core skeleton of
CC       terretonin (PubMed:25671343, PubMed:28759016). Trt14 catalyzes the D-
CC       ring expansion of terretonin involving intramolecular methoxy
CC       rearrangement as well as the hydrolysis of the expanded D-ring and the
CC       methyl ester moiety (PubMed:25671343, PubMed:28759016). Finally, the
CC       nonheme iron-dependent dioxygenase trt7 accomplishes the last two
CC       oxidation reactions steps to complete the biosynthesis of terretonin
CC       (PubMed:25671343). Terretonin C is produced via spontaneous
CC       decarboxylation of the terretonin precursor (PubMed:23116177). Another
CC       shunt product of the terretonin biosynthesis is dihydrofarnesyl-DMOA,
CC       derived from epoxyfarnesyl-DMOA through hydrolysis of the epoxide
CC       (PubMed:22549923, PubMed:22782788, PubMed:23116177).
CC       {ECO:0000269|PubMed:22549923, ECO:0000269|PubMed:22782788,
CC       ECO:0000269|PubMed:23116177, ECO:0000269|PubMed:25671343,
CC       ECO:0000269|PubMed:28759016}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:23116177}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the synthesis of terretonin
CC       (PubMed:23116177). {ECO:0000269|PubMed:23116177}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; CH476609; EAU29535.1; -; Genomic_DNA.
DR   RefSeq; XP_001209388.1; XM_001209388.1.
DR   AlphaFoldDB; Q0C898; -.
DR   SMR; Q0C898; -.
DR   STRING; 341663.Q0C898; -.
DR   EnsemblFungi; EAU29535; EAU29535; ATEG_10086.
DR   GeneID; 4319524; -.
DR   VEuPathDB; FungiDB:ATEG_10086; -.
DR   eggNOG; KOG0725; Eukaryota.
DR   HOGENOM; CLU_010194_1_0_1; -.
DR   OMA; EHAVAKC; -.
DR   OrthoDB; 1226147at2759; -.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           1..233
FT                   /note="Short chain dehydrogenase trt9"
FT                   /id="PRO_0000436598"
SQ   SEQUENCE   233 AA;  25103 MW;  543277C443E797FB CRC64;
     MALDQGAKVF GVDIAASPAP LANHPNYKFL KADLCDKQTP KDVVQACVKM FGGRIDGLLN
     IAGIMDSNQS VDSVSDEMWD RCIAVNLTAP IRLMREVIAI MREQKSGNIV NVASKAATSG
     AVSGVAYTAS KHGLVGATKN VAWRFKHDNI RCNAVCPGGV VGTGVHNEMD IQKWDKEAMK
     TMFLIHQSHS CDKDKGIGLR AEDIARPLLF LVSDRSKGIN GAILPIDNAW STI
 
 
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