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C1RL_HUMAN
ID   C1RL_HUMAN              Reviewed;         487 AA.
AC   Q9NZP8; Q53GX9;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 164.
DE   RecName: Full=Complement C1r subcomponent-like protein;
DE            Short=C1r-LP;
DE            Short=C1r-like protein;
DE            EC=3.4.21.-;
DE   AltName: Full=C1r-like serine protease analog protein;
DE            Short=CLSPa;
DE   Flags: Precursor;
GN   Name=C1RL; Synonyms=C1RL1, C1RLP, CLSPA;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, INDUCTION, AND VARIANT VAL-285.
RC   TISSUE=Dendritic cell;
RX   PubMed=15358180; DOI=10.1016/j.bbrc.2004.06.127;
RA   Lin N., Liu S., Li N., Wu P., An H., Yu Y., Wan T., Cao X.;
RT   "A novel human dendritic cell-derived C1r-like serine protease analog
RT   inhibits complement-mediated cytotoxicity.";
RL   Biochem. Biophys. Res. Commun. 321:329-336(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=15527420; DOI=10.1042/bj20041196;
RA   Ligoudistianou C., Xu Y., Garnier G., Circolo A., Volanakis J.E.;
RT   "A novel human complement-related protein, C1r-like protease (C1r-LP),
RT   specifically cleaves pro-C1s.";
RL   Biochem. J. 387:165-173(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RA   Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA   Tanaka A., Yokoyama S.;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16541075; DOI=10.1038/nature04569;
RA   Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA   Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA   Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA   Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA   Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA   Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA   Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA   Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA   Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA   Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA   Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA   Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA   Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA   Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA   Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA   Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA   Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA   David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA   D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA   Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA   Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA   Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA   LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA   Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA   Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA   Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA   Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA   Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA   Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA   Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA   Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA   Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA   Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA   Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA   Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA   Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA   Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA   Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA   Gibbs R.A.;
RT   "The finished DNA sequence of human chromosome 12.";
RL   Nature 440:346-351(2006).
RN   [5]
RP   FUNCTION, AND MUTAGENESIS OF SER-436.
RX   PubMed=15385675; DOI=10.1073/pnas.0405692101;
RA   Wicher K.B., Fries E.;
RT   "Prohaptoglobin is proteolytically cleaved in the endoplasmic reticulum by
RT   the complement C1r-like protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14390-14395(2004).
RN   [6]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-166; ASN-242 AND ASN-296.
RC   TISSUE=Plasma;
RX   PubMed=16335952; DOI=10.1021/pr0502065;
RA   Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA   Smith R.D.;
RT   "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT   hydrazide chemistry, and mass spectrometry.";
RL   J. Proteome Res. 4:2070-2080(2005).
RN   [7]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-147; ASN-242 AND ASN-296.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of multiple
RT   enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
RN   [8]
RP   GLYCOSYLATION AT ASN-242.
RX   PubMed=19139490; DOI=10.1074/mcp.m800504-mcp200;
RA   Jia W., Lu Z., Fu Y., Wang H.P., Wang L.H., Chi H., Yuan Z.F., Zheng Z.B.,
RA   Song L.N., Han H.H., Liang Y.M., Wang J.L., Cai Y., Zhang Y.K., Deng Y.L.,
RA   Ying W.T., He S.M., Qian X.H.;
RT   "A strategy for precise and large scale identification of core fucosylated
RT   glycoproteins.";
RL   Mol. Cell. Proteomics 8:913-923(2009).
CC   -!- FUNCTION: Mediates the proteolytic cleavage of HP/haptoglobin in the
CC       endoplasmic reticulum. {ECO:0000269|PubMed:15358180,
CC       ECO:0000269|PubMed:15385675, ECO:0000269|PubMed:15527420}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15358180}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in placenta, liver, kidney,
CC       pancreas, moderately in lung, spleen, prostate, ovary, colon, and PBL,
CC       and weakly in heart, skeletal muscle, thymus, testis, and small
CC       intestine. Expressed in PC-3 (prostate adenocarcinoma) and SK-OV-3
CC       (ovary adenocarcinoma) cells, but not in LoVo and HT-29 (colon
CC       adenocarcinoma), SMMC7721 (hepatocellular carcinoma), CaoV-3 (ovary
CC       adenocarcinoma), HeLa (cervix epithelioid carcinoma), MCF-7 (breast
CC       adenocarcinoma), U-251MG (glioma) or A-549 (lung carcinoma) cells.
CC       Widely expressed in myeloid leukemia cell lines, including K-562
CC       (chronic myelogenous leukemia), THP-1 (myelomonocytic leukemia), HL-60
CC       and NB4 (promyelocytic leukemia), and KG-1 (acute myelogenous leukemia)
CC       cells. Expressed mainly in the liver and in serum (at protein level).
CC       {ECO:0000269|PubMed:15358180, ECO:0000269|PubMed:15527420}.
CC   -!- INDUCTION: Up-regulated in monocytes and dendritic cells (DC)
CC       undergoing maturation or activation. {ECO:0000269|PubMed:15358180}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00274}.
CC   -!- CAUTION: Does not associate with the C1 complex. According to
CC       PubMed:15385675, doesn't cleave the proform of complement C1s.
CC       {ECO:0000305}.
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DR   EMBL; AF178985; AAF44349.1; -; mRNA.
DR   EMBL; AK222802; BAD96522.1; -; mRNA.
DR   EMBL; AC018653; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC094008; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC233309; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS8573.1; -.
DR   RefSeq; NP_001284569.1; NM_001297640.1.
DR   RefSeq; NP_057630.2; NM_016546.3.
DR   AlphaFoldDB; Q9NZP8; -.
DR   SMR; Q9NZP8; -.
DR   BioGRID; 119431; 10.
DR   IntAct; Q9NZP8; 1.
DR   STRING; 9606.ENSP00000266542; -.
DR   MEROPS; S01.189; -.
DR   GlyConnect; 1143; 11 N-Linked glycans (3 sites).
DR   GlyGen; Q9NZP8; 6 sites, 12 N-linked glycans (3 sites), 1 O-linked glycan (1 site).
DR   iPTMnet; Q9NZP8; -.
DR   PhosphoSitePlus; Q9NZP8; -.
DR   BioMuta; C1RL; -.
DR   DMDM; 182705204; -.
DR   CPTAC; non-CPTAC-1102; -.
DR   EPD; Q9NZP8; -.
DR   jPOST; Q9NZP8; -.
DR   MassIVE; Q9NZP8; -.
DR   PaxDb; Q9NZP8; -.
DR   PeptideAtlas; Q9NZP8; -.
DR   PRIDE; Q9NZP8; -.
DR   ProteomicsDB; 83474; -.
DR   Antibodypedia; 1734; 120 antibodies from 22 providers.
DR   DNASU; 51279; -.
DR   Ensembl; ENST00000266542.9; ENSP00000266542.4; ENSG00000139178.12.
DR   Ensembl; ENST00000671752.1; ENSP00000500804.1; ENSG00000288124.1.
DR   GeneID; 51279; -.
DR   KEGG; hsa:51279; -.
DR   MANE-Select; ENST00000266542.9; ENSP00000266542.4; NM_016546.4; NP_057630.2.
DR   UCSC; uc001qsn.4; human.
DR   CTD; 51279; -.
DR   DisGeNET; 51279; -.
DR   GeneCards; C1RL; -.
DR   HGNC; HGNC:21265; C1RL.
DR   HPA; ENSG00000139178; Tissue enhanced (liver).
DR   MIM; 608974; gene.
DR   neXtProt; NX_Q9NZP8; -.
DR   OpenTargets; ENSG00000139178; -.
DR   PharmGKB; PA134957759; -.
DR   VEuPathDB; HostDB:ENSG00000139178; -.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT00940000162495; -.
DR   HOGENOM; CLU_006842_0_0_1; -.
DR   InParanoid; Q9NZP8; -.
DR   OMA; NMFCVGD; -.
DR   OrthoDB; 518015at2759; -.
DR   PhylomeDB; Q9NZP8; -.
DR   TreeFam; TF330373; -.
DR   BRENDA; 3.4.21.41; 2681.
DR   PathwayCommons; Q9NZP8; -.
DR   SignaLink; Q9NZP8; -.
DR   SIGNOR; Q9NZP8; -.
DR   BioGRID-ORCS; 51279; 6 hits in 1073 CRISPR screens.
DR   ChiTaRS; C1RL; human.
DR   GenomeRNAi; 51279; -.
DR   Pharos; Q9NZP8; Tbio.
DR   PRO; PR:Q9NZP8; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; Q9NZP8; protein.
DR   Bgee; ENSG00000139178; Expressed in right lobe of liver and 98 other tissues.
DR   ExpressionAtlas; Q9NZP8; baseline and differential.
DR   Genevisible; Q9NZP8; HS.
DR   GO; GO:0072562; C:blood microparticle; IBA:GO_Central.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006958; P:complement activation, classical pathway; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0031638; P:zymogen activation; IBA:GO_Central.
DR   CDD; cd00041; CUB; 1.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   Gene3D; 2.60.120.290; -; 1.
DR   InterPro; IPR000859; CUB_dom.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR035914; Sperma_CUB_dom_sf.
DR   InterPro; IPR035976; Sushi/SCR/CCP_sf.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00431; CUB; 1.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00042; CUB; 1.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF49854; SSF49854; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   SUPFAM; SSF57535; SSF57535; 1.
DR   PROSITE; PS01180; CUB; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   1: Evidence at protein level;
KW   Complement pathway; Disulfide bond; Glycoprotein; Hydrolase; Immunity;
KW   Innate immunity; Protease; Reference proteome; Secreted; Serine protease;
KW   Signal; Sushi.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..487
FT                   /note="Complement C1r subcomponent-like protein"
FT                   /id="PRO_0000318678"
FT   DOMAIN          39..163
FT                   /note="CUB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00059"
FT   DOMAIN          165..230
FT                   /note="Sushi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT   DOMAIN          245..484
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        283
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        339
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        436
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        147
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19159218"
FT   CARBOHYD        166
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:16335952"
FT   CARBOHYD        242
FT                   /note="N-linked (GlcNAc...) (complex) asparagine"
FT                   /evidence="ECO:0000269|PubMed:16335952,
FT                   ECO:0000269|PubMed:19139490, ECO:0000269|PubMed:19159218"
FT   CARBOHYD        296
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:16335952,
FT                   ECO:0000269|PubMed:19159218"
FT   CARBOHYD        363
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        94..112
FT                   /evidence="ECO:0000250"
FT   DISULFID        195..228
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT   DISULFID        402..421
FT                   /evidence="ECO:0000250"
FT   DISULFID        432..462
FT                   /evidence="ECO:0000250"
FT   VARIANT         285
FT                   /note="I -> V (in dbSNP:rs3742089)"
FT                   /evidence="ECO:0000269|PubMed:15358180"
FT                   /id="VAR_038852"
FT   MUTAGEN         436
FT                   /note="S->A: Unable to cleave HP."
FT                   /evidence="ECO:0000269|PubMed:15385675"
FT   CONFLICT        94
FT                   /note="C -> R (in Ref. 3; BAD96522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        99
FT                   /note="V -> A (in Ref. 3; BAD96522)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        349
FT                   /note="I -> M (in Ref. 3; BAD96522)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   487 AA;  53498 MW;  6DE7CE9EA08C7990 CRC64;
     MPGPRVWGKY LWRSPHSKGC PGAMWWLLLW GVLQACPTRG SVLLAQELPQ QLTSPGYPEP
     YGKGQESSTD IKAPEGFAVR LVFQDFDLEP SQDCAGDSVT ISFVGSDPSQ FCGQQGSPLG
     RPPGQREFVS SGRSLRLTFR TQPSSENKTA HLHKGFLALY QTVAVNYSQP ISEASRGSEA
     INAPGDNPAK VQNHCQEPYY QAAAAGALTC ATPGTWKDRQ DGEEVLQCMP VCGRPVTPIA
     QNQTTLGSSR AKLGNFPWQA FTSIHGRGGG ALLGDRWILT AAHTIYPKDS VSLRKNQSVN
     VFLGHTAIDE MLKLGNHPVH RVVVHPDYRQ NESHNFSGDI ALLELQHSIP LGPNVLPVCL
     PDNETLYRSG LLGYVSGFGM EMGWLTTELK YSRLPVAPRE ACNAWLQKRQ RPEVFSDNMF
     CVGDETQRHS VCQGDSGSVY VVWDNHAHHW VATGIVSWGI GCGEGYDFYT KVLSYVDWIK
     GVMNGKN
 
 
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