ID   TRUA_LEPIN              Reviewed;         207 AA.
AC   Q8CXS6;
DT   16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA pseudouridine(38-40) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase I;
DE   AltName: Full=tRNA-uridine isomerase I;
GN   Name=truA; OrderedLocusNames=LA_2740;
OS   Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain
OS   56601).
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=189518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=56601;
RX   PubMed=12712204; DOI=10.1038/nature01597;
RA   Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X.,
RA   Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X.,
RA   Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H., Yin H.-F., Zhang Y., Zhu G.-F.,
RA   Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y.,
RA   Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I.,
RA   Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P.;
RT   "Unique physiological and pathogenic features of Leptospira interrogans
RT   revealed by whole-genome sequencing.";
RL   Nature 422:888-893(2003).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the
CC       anticodon stem and loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000305}.
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DR   EMBL; AE010300; AAN49939.1; -; Genomic_DNA.
DR   RefSeq; NP_712921.1; NC_004342.2.
DR   AlphaFoldDB; Q8CXS6; -.
DR   SMR; Q8CXS6; -.
DR   STRING; 189518.LA_2740; -.
DR   EnsemblBacteria; AAN49939; AAN49939; LA_2740.
DR   KEGG; lil:LA_2740; -.
DR   PATRIC; fig|189518.3.peg.2722; -.
DR   HOGENOM; CLU_014673_0_1_12; -.
DR   InParanoid; Q8CXS6; -.
DR   OMA; RRNVWHV; -.
DR   Proteomes; UP000001408; Chromosome I.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR   Gene3D; 3.30.70.660; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..207
FT                   /note="tRNA pseudouridine synthase A"
FT                   /id="PRO_0000057401"
FT   ACT_SITE        26
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         52
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   207 AA;  24296 MW;  DB9FF94535392134 CRC64;
     MIVNFKTQRV IQNFSKFLLS LNAITDSGLS ILNMTEVDEN FDSRFSCNSR EYEYWILNTK
     YPRPTWKNRT FWYQHRIDVP RLEAELELLK GEHDFRSLAK VASLKGRSTV RTILDVKLER
     SLELEGLLKV KIRANGFLHN MIRILTGTLL EISNGKRKDT NVLEILSSKD RTIAGITLPP
     YGLYFIRAYY DSYPKIDFMY SHLDFLK