TRUA_MYCGE
ID TRUA_MYCGE Reviewed; 244 AA.
AC P47428; Q49300;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=tRNA pseudouridine synthase A;
DE EC=5.4.99.12;
DE AltName: Full=tRNA pseudouridine(38-40) synthase;
DE AltName: Full=tRNA pseudouridylate synthase I;
DE AltName: Full=tRNA-uridine isomerase I;
GN Name=truA; Synonyms=hisT; OrderedLocusNames=MG182;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102 AND 129-244.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the
CC anticodon stem and loop of transfer RNAs. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000305}.
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DR EMBL; L43967; AAC71401.1; -; Genomic_DNA.
DR EMBL; U02176; AAD12460.1; -; Genomic_DNA.
DR EMBL; U02100; AAD12372.1; -; Genomic_DNA.
DR PIR; B64220; B64220.
DR RefSeq; WP_009885867.1; NZ_AAGX01000007.1.
DR AlphaFoldDB; P47428; -.
DR SMR; P47428; -.
DR STRING; 243273.MG_182; -.
DR EnsemblBacteria; AAC71401; AAC71401; MG_182.
DR KEGG; mge:MG_182; -.
DR eggNOG; COG0101; Bacteria.
DR HOGENOM; CLU_014673_0_1_14; -.
DR OMA; FLYGMVR; -.
DR OrthoDB; 1075262at2; -.
DR BioCyc; MGEN243273:G1GJ2-206-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02570; PseudoU_synth_EcTruA; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..244
FT /note="tRNA pseudouridine synthase A"
FT /id="PRO_0000057409"
FT ACT_SITE 52
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 110
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 13
FT /note="S -> I (in Ref. 2; AAD12460)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 244 AA; 27597 MW; AA1071218B916492 CRC64;
MARYLGIVSY DGSYFKGWAI QPNLATIQGL LEQSFSLIIG RKIKVIGSGR TDKGVHAINQ
TFHVDINGEI NLNLLIRKIN QLIKPHCIVK TLVLVNDSFH ARFQVKTKVY EYLINCGNLN
PLQFNYVWQL NQQLDLEKLK ADATLFLGKK NFLSFSSSIH TDSIRTISKI TIQKETNQLV
RLTFFGSGFL RSQVRMIVAC LVNLNTNKMA LETVAKLFEH PKKGSCVVKA PSCGLYLKTV
VYEK