ID   C20AA_BACUF             Reviewed;         753 AA.
AC   O32321;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Pesticidal crystal protein Cry20Aa;
DE   AltName: Full=86 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXXA(a);
GN   Name=cry20Aa; Synonyms=cryXXA(a);
OS   Bacillus thuringiensis subsp. fukuokaensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=132265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9406385; DOI=10.1128/aem.63.12.4664-4670.1997;
RA   Lee H.-K., Gill S.S.;
RT   "Molecular cloning and characterization of a novel mosquitocidal protein
RT   gene from Bacillus thuringiensis subsp. fukuokaensis.";
RL   Appl. Environ. Microbiol. 63:4664-4670(1997).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of mosquitos. Active against Aedes aegypti and Culex
CC       quinquefasciatus larvae.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- PTM: Has low mosquitocidal activity probably due to rapid proteolysis
CC       to inactive 56 kDa and 43 kDa proteins.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; U82518; AAB93476.1; -; Genomic_DNA.
DR   AlphaFoldDB; O32321; -.
DR   SMR; O32321; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..753
FT                   /note="Pesticidal crystal protein Cry20Aa"
FT                   /id="PRO_0000174096"
FT   REGION          680..721
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   753 AA;  86139 MW;  CE3DED573EA41D66 CRC64;
     MNPYQNNDEI VDVPENYDNN LNRYPYANDP NVAMQNTNYK DWMNGYEEIN PSSITAILAS
     IGILNRVIAL TGVLGNTQEV ISIIQDALGF IRNGTGNELL IHVEQLIQQT LATQYRSAAT
     GAIYGISRSY DNYLMFFRQW ERNRTRENGQ QVESAFTTIN TLCINALAPQ ASLSRRGFET
     LLLPNYAMAA NFHLLLLRDA VLYRNQWLSN SISTANVNLN ILRAAINEYI THCTRWYQDG
     LNRFDRSSRA NMNEWRRFNA YRRDMTLSVL DFATVFPTYD PVLFPAATNV ELTRVVYTDP
     IVMAGGRTAI PGFTRMENLV NSASRVSFLN QMNIYTSFYF RPHNIPRYYW SGNQNFLSNG
     TSNLYGYRSD GRTTFNVSNI DIFRVNMTTH IGGAFTDDYR GLHRAEFIGA NTQNNQRTSL
     LYSVEIPSSH FRFENHTVFL PGESGLEPNE RNYTHRLFQM MNEVSVNPNA RGRVFLHAWT
     HRSLRRTNGL RSDQILQIPA VKTISNGGDR AVVLNYGENI MKLDNLTTGL SYKLTAVDSE
     ASNTRFIVRV RYASMNNNKL NLVLNGAQIA SLNVEHTVQR GGSLTDLQYG NFKYATFAGN
     FKMGSQSILG IFKEIPNIDF VLDKIELIPS NFMSSLEQTQ NYNTYNQDTI YTHNQGYDTY
     DQNSSGMYHQ SYNNYDQNMD TTYQPSYDNY NQNASGTYDD GYNPNASDSY DQSYTNNYSQ
     NTNSMYDQGY YNNNYDQHSG CTCNQGYDNN YLK