ID   TRUA_SYNP6              Reviewed;         294 AA.
AC   O24712; Q5N0U0;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=tRNA pseudouridine synthase A {ECO:0000255|HAMAP-Rule:MF_00171};
DE            EC=5.4.99.12 {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA pseudouridylate synthase I {ECO:0000255|HAMAP-Rule:MF_00171};
DE   AltName: Full=tRNA-uridine isomerase I {ECO:0000255|HAMAP-Rule:MF_00171};
GN   Name=truA {ECO:0000255|HAMAP-Rule:MF_00171}; Synonyms=hisT;
GN   OrderedLocusNames=syc1890_d;
OS   Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis
OS   nidulans).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=269084;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27144 / PCC 6301 / SAUG 1402/1;
RX   PubMed=17211581; DOI=10.1007/s11120-006-9122-4;
RA   Sugita C., Ogata K., Shikata M., Jikuya H., Takano J., Furumichi M.,
RA   Kanehisa M., Omata T., Sugiura M., Sugita M.;
RT   "Complete nucleotide sequence of the freshwater unicellular cyanobacterium
RT   Synechococcus elongatus PCC 6301 chromosome: gene content and
RT   organization.";
RL   Photosyn. Res. 93:55-67(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
RX   PubMed=9300823; DOI=10.1016/s0378-1119(97)00169-8;
RA   Sugita M., Sugishita H., Fujishiro T., Tsuboi M., Sugita C., Endo T.,
RA   Sugiura M.;
RT   "Organization of a large gene cluster encoding ribosomal proteins in the
RT   cyanobacterium Synechococcus sp. strain PCC 6301: comparison of gene
RT   clusters among cyanobacteria, eubacteria and chloroplast genomes.";
RL   Gene 195:73-79(1997).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the
CC       anticodon stem and loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_00171}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC         Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00171};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00171}.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000255|HAMAP-Rule:MF_00171}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA22474.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP008231; BAD80080.1; -; Genomic_DNA.
DR   EMBL; AB000111; BAA22474.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011244200.1; NC_006576.1.
DR   AlphaFoldDB; O24712; -.
DR   SMR; O24712; -.
DR   STRING; 269084.syc1890_d; -.
DR   EnsemblBacteria; BAD80080; BAD80080; syc1890_d.
DR   KEGG; syc:syc1890_d; -.
DR   eggNOG; COG0101; Bacteria.
DR   OMA; FLYGMVR; -.
DR   Proteomes; UP000001175; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02570; PseudoU_synth_EcTruA; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..294
FT                   /note="tRNA pseudouridine synthase A"
FT                   /id="PRO_0000057471"
FT   ACT_SITE        64
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00171"
FT   BINDING         122
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00171"
FT   CONFLICT        69
FT                   /note="A -> R (in Ref. 2; BAA22474)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   294 AA;  32905 MW;  74D2EF777E779194 CRC64;
     MAIAEEPAQA PVIQRIALVI QYLGQGFCGW QRQPRQRSVQ GELESAIAAV VGHPVSVQSA
     GRTDTGVHAA AQVAHFETTS PIPAHRWPSV LNGRLTPDLN IRAAAIVPAN WHARFSASYR
     RYRYTIYTDP CPNLFLNSYV WHYYQAPLCE NQMQAALQTL VGYHHLAAFQ RSGSKRQHAW
     VHVQDAWVRR RDSLIEIEVQ ASGFLYGMIR LLVGLLVQVG EGSRSLESFT DIWVNQRRDR
     VRHAAPPQGL CLLRIGYPDS PFPVDAWFDT QPLFVLPSSR NDSESLSPCA VSLG