TRUA_THET8
ID TRUA_THET8 Reviewed; 253 AA.
AC Q5SHU9;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=tRNA pseudouridine synthase A {ECO:0000255|HAMAP-Rule:MF_00171};
DE EC=5.4.99.12 {ECO:0000255|HAMAP-Rule:MF_00171};
DE AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000255|HAMAP-Rule:MF_00171};
DE AltName: Full=tRNA pseudouridylate synthase I {ECO:0000255|HAMAP-Rule:MF_00171};
DE AltName: Full=tRNA-uridine isomerase I {ECO:0000255|HAMAP-Rule:MF_00171};
GN Name=truA {ECO:0000255|HAMAP-Rule:MF_00171}; OrderedLocusNames=TTHA1631;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 1-249, AND SUBUNIT.
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RX PubMed=17114947; DOI=10.4161/rna.3.3.3286;
RA Dong X., Bessho Y., Shibata R., Nishimoto M., Shirouzu M., Kuramitsu S.,
RA Yokoyama S.;
RT "Crystal structure of tRNA pseudouridine synthase TruA from Thermus
RT thermophilus HB8.";
RL RNA Biol. 3:115-122(2006).
CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in the
CC anticodon stem and loop of transfer RNAs. {ECO:0000255|HAMAP-
CC Rule:MF_00171}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00171};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00171,
CC ECO:0000269|PubMed:17114947}.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000255|HAMAP-Rule:MF_00171}.
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DR EMBL; AP008226; BAD71454.1; -; Genomic_DNA.
DR RefSeq; WP_011173668.1; NC_006461.1.
DR RefSeq; YP_144897.1; NC_006461.1.
DR PDB; 1VS3; X-ray; 2.25 A; A/B=1-249.
DR PDBsum; 1VS3; -.
DR AlphaFoldDB; Q5SHU9; -.
DR SMR; Q5SHU9; -.
DR STRING; 300852.55773013; -.
DR EnsemblBacteria; BAD71454; BAD71454; BAD71454.
DR GeneID; 3168739; -.
DR KEGG; ttj:TTHA1631; -.
DR PATRIC; fig|300852.9.peg.1601; -.
DR eggNOG; COG0101; Bacteria.
DR HOGENOM; CLU_014673_0_1_0; -.
DR OMA; FLYGMVR; -.
DR PhylomeDB; Q5SHU9; -.
DR EvolutionaryTrace; Q5SHU9; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd02570; PseudoU_synth_EcTruA; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..253
FT /note="tRNA pseudouridine synthase A"
FT /id="PRO_0000057476"
FT ACT_SITE 52
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00171"
FT BINDING 110
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00171"
FT STRAND 2..10
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 12..14
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 16..19
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 28..35
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 36..39
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 46..49
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 56..66
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 72..74
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 75..82
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 87..95
FT /evidence="ECO:0007829|PDB:1VS3"
FT TURN 101..104
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 106..116
FT /evidence="ECO:0007829|PDB:1VS3"
FT TURN 122..127
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 128..131
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 137..147
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 148..152
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 153..156
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 165..177
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 179..191
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 197..210
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 211..213
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 215..217
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 218..224
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:1VS3"
FT HELIX 236..238
FT /evidence="ECO:0007829|PDB:1VS3"
FT STRAND 239..245
FT /evidence="ECO:0007829|PDB:1VS3"
SQ SEQUENCE 253 AA; 28134 MW; 84848C64A5F972BF CRC64;
MRRLLLLCEY DGTLFAGLQR QGRGLRTVQG ELERALPGIG ALPKAVAAGR TDAGVHALAM
PFHVDVESAI PVEKVPEALN RLLPEDLKVV GAREVAPDFH ARKDALWRAY RYRILVRPHP
SPLLRHRALW VRRPLDLEAM EEALSLLLGR HNFLGFAKEE TRPGERELLE ARLQVAEGEA
GLEVRLYFRG KSFLRGQVRG MVGTLLEVGL GKRPPESLKA ILKTADRRLA GPTAPAHGLY
FVEAAYPEEK LSP
