ID   C21AA_BACTU             Reviewed;        1167 AA.
AC   P56956;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Pesticidal crystal protein Cry21Aa;
DE   AltName: Full=132 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXXIA(a);
GN   Name=cry21Aa; Synonyms=cryXXIA(a);
OS   Bacillus thuringiensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-18681 / PS167P;
RA   Payne J.M., Narva K.E., Fu J.M.;
RT   "Bacillus thuringiensis genes encoding nematode-active toxins.";
RL   Patent number US5589382, 31-DEC-1996.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL B-21544 / PS178D4;
RA   Feitelson J.S.;
RT   "Identification of, and uses for, nematicidal Bacillus thuringiensis genes,
RT   toxins, and isolates.";
RL   Patent number US5670365, 23-SEP-1997.
CC   -!- FUNCTION: Endotoxin with nematicidal activity.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; I32932; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR   EMBL; I66477; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR   AlphaFoldDB; P56956; -.
DR   SMR; P56956; -.
DR   PRIDE; P56956; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1167
FT                   /note="Pesticidal crystal protein Cry21Aa"
FT                   /id="PRO_0000174097"
FT   VARIANT         237
FT                   /note="G -> A (in strain: PS178D4)"
FT   VARIANT         477
FT                   /note="T -> A (in strain: PS178D4)"
FT   VARIANT         586
FT                   /note="Q -> K (in strain: PS178D4)"
FT   VARIANT         992..994
FT                   /note="ASD -> HVT (in strain: PS178D4)"
FT   VARIANT         1016
FT                   /note="G -> D (in strain: PS178D4)"
FT   VARIANT         1050
FT                   /note="L -> F (in strain: PS178D4)"
FT   CONFLICT        1094..1118
FT                   /note="GRHRIQTACTWKRQRNSYRSTWRKR -> EDTEYKLRVRGKGKGTVTVQHGE
FT                   EE (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1167 AA;  131658 MW;  47A1ED4EBDC0C645 CRC64;
     MTNPTILYPS YHNVLAHPIR LDSFFDPFVE TFKDLKGAWE EFGKTGYMDP LKQHLQIAWD
     TSQNGTVDYL ALTKASISLI GLIPGADAVV PFINMFVDFI FPKLFGRGSQ QNAQAQFFEL
     IIEKVKELVD EDFRNFTLNN LLNYLDGMQT ALSHFQNDVQ IAICQGEQPG LMLDQTPTAC
     TPTTDHLISV RESFKDARTT IETALPHFKN PMLSTNDNTP DFNSDTVLLT LPMYTTGATL
     NLILHQGYIQ FAERWKSVNY DESFINQTKV DLQRRIQDYS TTVSTTFEKF KPTLNPSNKE
     SVNKYNRYVR SMTLQSLDIA ATWPTLDNVN YPSNVDIQLD QTRLVFSDVA GPWEGNDNIT
     SNIIDVLTPI NTGIGFQESS DLRKFTYPRI ELQSMQFHGQ YVNSKSVEHC YSDGLKLNYK
     NKTITAGVSN IDESNQNNKH NYGPVINSPI TDINVNSQNS QYLDLNSVMV NGGQKVTGCS
     PLSSNGNSNN AALPNQKINV IYSVQSNDKP EKHADTYRKW GYMSSHIPYD LVPENVIGDI
     DPDTKQPSLL LKGFPAEKGY GDSIAYVSEP LNGANAVKLT SYQVLQMEVT NQTTQKYRIR
     IRYATGGDTA ASIWFHIIGP SGNDLTNEGH NFSSVSSRNK MFVQGNNGKY VLNILTDSIE
     LPSGQQTILI QNTNSQDLFL DRIEFISLPS TSTPTSTNFV EPESLEKIIN QVNQLFSSSS
     QTELAHTVSD YKIDQVVLKV NALSDDVFGV EKKALRKLVN QAKQLSKARN VLVGGNFEKG
     HEWALSREAT MVANHELFKG DHLLLPPPTL YPSYAYQKID ESKLKSNTRY TVSGFIAQSE
     HLEVVVSRYG KEVHDMLDIP YEEALPISSD ESPNCCKPAA CQCSSCDGSQ SDSHFFSYSI
     DVGSLQSDVN LGIEFGLRIA KPNGFAKISN LEIKEDRPLT EKEIKKVQRK EQKWKKAFNQ
     EQAEVATTLQ PTLDQINALY QNEDWNGSVH PASDYQHLSA VVVPTLPKQR HWFMEGREGE
     HVVLTQQFQQ ALDRAFQQIE EQNLIHNGNL ANGLTDWTVT GDAQLTIFDE DPVLELAHWD
     ASISQTIEIM DFEGRHRIQT ACTWKRQRNS YRSTWRKRLE TMTFNTTSFT TQEQTFYFEG
     DTVDVHVQSE NNTFLIDSVE LIEIIEE