ID   C23L_VACCC              Reviewed;         244 AA.
AC   P21090;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   29-SEP-2021, entry version 79.
DE   RecName: Full=Inactive chemokine-binding protein;
DE            Short=vCKBP;
GN   Name=C23L;
GN   and
GN   Name=B29R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
CC   -!- FUNCTION: The protein is truncated in this vaccinal strain and
CC       presumably inactive, because the lack of signal peptide prevents the
CC       protein of being secreted. In the other strains inhibits host immune
CC       defense by binding to host chemokines. Binds host CC chemokines (beta
CC       chemokines) such as RANTES with high affinity, but not CXC or C
CC       chemokines (alpha and gamma chemokines) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}. Note=the wild-type
CC       protein is secreted, but this strain encodes a truncated form which
CC       lacks the signal peptide. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae chemokine-binding protein family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48229.1; -; Genomic_DNA.
DR   EMBL; M35027; AAA47970.1; -; Genomic_DNA.
DR   PIR; I33172; A42529.
DR   BMRB; P21090; -.
DR   SMR; P21090; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.240.10; -; 1.
DR   InterPro; IPR009173; Chemkine-bd_vir.
DR   InterPro; IPR003184; Orthopox_35kDa.
DR   InterPro; IPR036540; Pox_vCCI-like_sf.
DR   Pfam; PF02250; Orthopox_35kD; 1.
DR   PIRSF; PIRSF003696; VAC_C23L; 1.
DR   SUPFAM; SSF49889; SSF49889; 1.
PE   3: Inferred from homology;
KW   Early protein; Host cytoplasm; Reference proteome.
FT   CHAIN           1..244
FT                   /note="Inactive chemokine-binding protein"
FT                   /id="PRO_0000099422"
FT   REGION          1..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        35..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        56..74
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   244 AA;  26385 MW;  5DFFD64E7DCA6B93 CRC64;
     MHVPASLQQS SSSSSSCTEE ENKHHMGIDV IIKVTKQDQT PTNDKICQSV TEITESESDP
     DPEVESEDDS TSVEDVDPPT TYYSIIGGGL RMNFGFTKCP QIKSISESAD GNTVNARLSS
     VSPGQGKDSP AITREEALAM IKDCEVSIDI RCSEEEKDSD IKTHPVLGSN ISHKKVSYED
     IIGSTIVDTK CVKNLEFSVR IGDMCKESSE LEVKDGFKYV DGSASEGATD DTSLIDSTKL
     KACV