ID   TRUB2_XENTR             Reviewed;         325 AA.
AC   Q5XGG2;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Pseudouridylate synthase TRUB2, mitochondrial {ECO:0000305};
DE            EC=5.4.99.- {ECO:0000250|UniProtKB:O95900};
DE   AltName: Full=TruB pseudouridine synthase homolog 2 {ECO:0000250|UniProtKB:O95900};
DE   AltName: Full=tRNA pseudouridine 55 synthase TRUB2 {ECO:0000305};
DE            Short=Psi55 synthase TRUB2 {ECO:0000305};
DE            EC=5.4.99.25 {ECO:0000250|UniProtKB:O95900};
DE   Flags: Precursor;
GN   Name=Trub2 {ECO:0000250|UniProtKB:O95900};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Minor enzyme contributing to the isomerization of uridine to
CC       pseudouridine (pseudouridylation) of specific mitochondrial mRNAs (mt-
CC       mRNAs) such as COXI and COXIII mt-mRNAs, modulating the efficiency of
CC       mitochondrial protein synthesis without changes in transcript abundance
CC       or stability (By similarity). Also catalyzes pseudouridylation of some
CC       tRNAs, including synthesis of pseudouridine(55) from uracil-55, in the
CC       psi GC loop of a subset of tRNAs (By similarity).
CC       {ECO:0000250|UniProtKB:O95900}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in mRNA = a pseudouridine in mRNA;
CC         Xref=Rhea:RHEA:56644, Rhea:RHEA-COMP:14658, Rhea:RHEA-COMP:14659,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000250|UniProtKB:O95900};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000250|UniProtKB:O95900};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42533;
CC         Evidence={ECO:0000250|UniProtKB:O95900};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix
CC       {ECO:0000250|UniProtKB:O95900}. Note=Localizes to mitochondrial RNA
CC       granules, platforms for post-transcriptional RNA modification and
CC       ribosome assembly. {ECO:0000250|UniProtKB:O95900}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC       {ECO:0000305}.
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DR   EMBL; BC084478; AAH84478.1; -; mRNA.
DR   RefSeq; NP_001011086.1; NM_001011086.1.
DR   AlphaFoldDB; Q5XGG2; -.
DR   SMR; Q5XGG2; -.
DR   STRING; 8364.ENSXETP00000008598; -.
DR   PaxDb; Q5XGG2; -.
DR   DNASU; 496499; -.
DR   GeneID; 496499; -.
DR   KEGG; xtr:496499; -.
DR   CTD; 26995; -.
DR   Xenbase; XB-GENE-996199; trub2.
DR   eggNOG; KOG2559; Eukaryota.
DR   InParanoid; Q5XGG2; -.
DR   OrthoDB; 1535798at2759; -.
DR   Proteomes; UP000008143; Chromosome 8.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0009982; F:pseudouridine synthase activity; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR039048; Trub2.
DR   PANTHER; PTHR13195; PTHR13195; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Mitochondrion; mRNA processing; Reference proteome;
KW   Transit peptide; tRNA processing.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..325
FT                   /note="Pseudouridylate synthase TRUB2, mitochondrial"
FT                   /id="PRO_0000252093"
FT   REGION          292..325
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        101
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y606"
SQ   SEQUENCE   325 AA;  36099 MW;  6C2A2CDE81AEC293 CRC64;
     MAQFAPGVFR TLHGLFAVYK PPGVHWKSVR DTVETNLLKE LNALKQRPPR QQIRFLPAGT
     EGSNGLEVTR VPSAVPVLAD HVLVKGPAFT HIRVGTGHRL DIQSSGVFVL GIGHGNKLLK
     DMYNSHFTRD YTVRGMLGKA TEDFTELGKT IEKTTYDHIT REKLERILAV IQGTNQKALI
     THSHLDLQSQ EAYDLAVQGK LRPMVKSPPI ILGIRCLEFS PPEFTLEIQC MHETQQYLRK
     MIHEIGLELR SSAVCTQVRR SRDGPFTVDC SLLRTQWDLG SIQGAIRECR AQTEGVSRGN
     PDREAAEGPI PGPSRGAEGE GELRA