ID   C23L_VACCL              Reviewed;         258 AA.
AC   P19063;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Chemokine-binding protein;
DE            Short=vCKBP;
DE   AltName: Full=35 kDa protein;
DE   Flags: Precursor;
GN   Name=C23L;
GN   and
GN   Name=B29R;
OS   Vaccinia virus (strain Lister) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10252;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2212991; DOI=10.1099/0022-1317-71-9-2013;
RA   Patel A.H., Gaffney D.F., Subak-Sharpe J.H., Stow N.D.;
RT   "DNA sequence of the gene encoding a major secreted protein of vaccinia
RT   virus, strain Lister.";
RL   J. Gen. Virol. 71:2013-2021(1990).
RN   [2]
RP   CHARACTERIZATION, FUNCTION, AND CHEMOKINE-BINDING.
RX   PubMed=9551896;
RA   Alcami A., Symons J.A., Collins P.D., Williams T.J., Smith G.L.;
RT   "Blockade of chemokine activity by a soluble chemokine binding protein from
RT   vaccinia virus.";
RL   J. Immunol. 160:624-633(1998).
CC   -!- FUNCTION: Inhibits host immune defense by binding to host chemokines.
CC       Binds host CC chemokines (beta chemokines) such as RANTES with high
CC       affinity, but not CXC or C chemokines (alpha and gamma chemokines).
CC       {ECO:0000269|PubMed:9551896}.
CC   -!- SUBUNIT: Binds to host CC chemokines, such as RANTES/CCL5, MIP-
CC       1alpha/CCL3, MCP-1/CCL2 and eotaxin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the poxviridae chemokine-binding protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00612; BAA00487.1; -; Genomic_DNA.
DR   PIR; A36640; WMVZ35.
DR   BMRB; P19063; -.
DR   SMR; P19063; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0039553; P:suppression by virus of host chemokine activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.240.10; -; 1.
DR   InterPro; IPR009173; Chemkine-bd_vir.
DR   InterPro; IPR003184; Orthopox_35kDa.
DR   InterPro; IPR036540; Pox_vCCI-like_sf.
DR   Pfam; PF02250; Orthopox_35kD; 1.
DR   PIRSF; PIRSF003696; VAC_C23L; 1.
DR   SUPFAM; SSF49889; SSF49889; 1.
PE   1: Evidence at protein level;
KW   Early protein; Host-virus interaction;
KW   Inhibition of host chemokines by virus; Secreted; Signal;
KW   Viral immunoevasion.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..258
FT                   /note="Chemokine-binding protein"
FT                   /id="PRO_0000040612"
FT   REGION          65..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        70..88
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   258 AA;  27831 MW;  0909F9B6B68F7BFF CRC64;
     MKQYIVLACM CLAAAAMPAS LQQSSSSSSS CTEEENKHHM GIDVIIKVTK QDQTPTNDKI
     CQSVTEITES ESDPDPEVES EDDSTSVEDV DPPTTYYSII GGGLRMNFGF TKCPQIKSIS
     ESADGNTVNA RLSSVSPGQG KDSPAITREE ALAMIKDCEV SIDIRCSEEE KDSDIKTHPV
     LGSNISHKKV SYEDIIGSTI VDTKCVKNLE FSVRIGDMCK ESSELEVKDG FKYVDGSASE
     GATDDTSLID STKLKACV