ID   C23L_VAR67              Reviewed;         253 AA.
AC   P34016;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Chemokine-binding protein;
DE            Short=vCKBP;
DE   AltName: Full=35 kDa protein;
DE   Flags: Precursor;
GN   Name=C23L;
GN   and
GN   Name=B29R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=1666548;
RA   Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA   Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA   Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT   "Creation of a clone library of fragments from the natural variola virus
RT   and study of the structural and functional organization of viral genes from
RT   a circle of hosts.";
RL   Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=India-1967 / Isolate Ind3;
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Inhibits host immune defense by binding to host chemokines.
CC       Binds host CC chemokines (beta chemokines) such as RANTES with high
CC       affinity, but not CXC or C chemokines (alpha and gamma chemokines) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Binds to host CC chemokines, such as RANTES/CCL5, MIP-
CC       1alpha/CCL3, MCP-1/CCL2 and eotaxin. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae chemokine-binding protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X69198; CAA49138.1; -; Genomic_DNA.
DR   EMBL; X67117; CAA47541.1; -; Genomic_DNA.
DR   PIR; S46889; S46889.
DR   RefSeq; NP_042241.1; NC_001611.1.
DR   SMR; P34016; -.
DR   PRIDE; P34016; -.
DR   GeneID; 1486428; -.
DR   KEGG; vg:1486428; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0039553; P:suppression by virus of host chemokine activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.240.10; -; 1.
DR   InterPro; IPR009173; Chemkine-bd_vir.
DR   InterPro; IPR003184; Orthopox_35kDa.
DR   InterPro; IPR036540; Pox_vCCI-like_sf.
DR   Pfam; PF02250; Orthopox_35kD; 1.
DR   PIRSF; PIRSF003696; VAC_C23L; 1.
DR   SUPFAM; SSF49889; SSF49889; 1.
PE   3: Inferred from homology;
KW   Early protein; Host-virus interaction;
KW   Inhibition of host chemokines by virus; Reference proteome; Secreted;
KW   Signal; Viral immunoevasion.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..253
FT                   /note="Chemokine-binding protein"
FT                   /id="PRO_0000040613"
FT   REGION          62..87
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        67..83
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   253 AA;  27465 MW;  F639F91E55F31910 CRC64;
     MKQYIVLACM CLAAAAMPAS LQQSSSSCTE EENKHYMGID VIIKVTKQDQ TPTNDKICQS
     VTEITESESD PEVESEDDST SVEDVDPPTT YYSIIGGGLR MNFGFTKCPQ IKSISESANG
     NAVNARLSSV PLGQGKDSPA ITRAEALAMI KDCELSIDIR CSEEEKDSDI QTHPVLESNI
     SHKKVSYEDI IGSTIVDTKC VKNLEFSVRI GDMCKESSDL EVKDGFKYVD GSVSEGVTDD
     TSLIDSTKLK SCV