C2512_DANRE
ID C2512_DANRE Reviewed; 276 AA.
AC Q1LX59;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Cholesterol 25-hydroxylase-like protein 1, member 2 {ECO:0000312|ZFIN:ZDB-GENE-050419-90};
DE EC=1.14.99.-;
GN Name=ch25hl1.2 {ECO:0000312|ZFIN:ZDB-GENE-050419-90};
GN ORFNames=si:dkey-24l11.9;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
CC -!- FUNCTION: May catalyze the formation of 25-hydroxycholesterol from
CC cholesterol. {ECO:0000250|UniProtKB:Q9Z0F5}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000250|UniProtKB:Q9Z0F5};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q9Z0F5}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9Z0F5}.
CC -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000255}.
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DR EMBL; BX470164; CAK10818.1; -; Genomic_DNA.
DR RefSeq; NP_001076393.1; NM_001082924.1.
DR AlphaFoldDB; Q1LX59; -.
DR STRING; 7955.ENSDARP00000089118; -.
DR PaxDb; Q1LX59; -.
DR Ensembl; ENSDART00000098347; ENSDARP00000089118; ENSDARG00000068138.
DR GeneID; 798384; -.
DR KEGG; dre:798384; -.
DR CTD; 798384; -.
DR ZFIN; ZDB-GENE-050419-90; ch25hl1.2.
DR eggNOG; KOG0873; Eukaryota.
DR GeneTree; ENSGT00940000163793; -.
DR HOGENOM; CLU_047036_5_1_1; -.
DR InParanoid; Q1LX59; -.
DR OMA; CDIMGDR; -.
DR OrthoDB; 1493916at2759; -.
DR PhylomeDB; Q1LX59; -.
DR TreeFam; TF314256; -.
DR PRO; PR:Q1LX59; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 18.
DR Bgee; ENSDARG00000068138; Expressed in brain and 6 other tissues.
DR ExpressionAtlas; Q1LX59; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000254; F:C-4 methylsterol oxidase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0008395; F:steroid hydroxylase activity; IBA:GO_Central.
DR GO; GO:0008203; P:cholesterol metabolic process; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR InterPro; IPR006694; Fatty_acid_hydroxylase.
DR Pfam; PF04116; FA_hydroxylase; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycoprotein; Iron; Lipid biosynthesis;
KW Lipid metabolism; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW Reference proteome; Steroid biosynthesis; Steroid metabolism;
KW Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT CHAIN 1..276
FT /note="Cholesterol 25-hydroxylase-like protein 1, member 2"
FT /id="PRO_0000385451"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 134..265
FT /note="Fatty acid hydroxylase"
FT /evidence="ECO:0000255"
FT MOTIF 144..148
FT /note="Histidine box-1"
FT /evidence="ECO:0000255"
FT MOTIF 159..163
FT /note="Histidine box-2"
FT /evidence="ECO:0000255"
FT MOTIF 240..246
FT /note="Histidine box-3"
FT /evidence="ECO:0000255"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 164
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 276 AA; 32063 MW; 0915C436C5137A25 CRC64;
MTLAHIFQTI WDFGTKDSVL QPIWDYVRLN HSETLRSPLF PVVLTVSSYF VLVLPYLSCD
ILGRKWPAIY RYKIQPDKLP TTAMLLHCSG VTLYNHILLV IPAAVAQWMW RPPIPLPEQA
PTLLELVGGV TGNLLLFDLQ YFIWHFLHHK IRWLYVTFHA IHHNYSAPFA LATQCLGGWE
LVTVGFWTTL NPVLLRCHLL TTWMFMVVHV YVSVEDHCGY DFPWSTSRLI PFGVYGGPSK
HDVHHQKPNT NFAPHFSHWD KMFGTHADFR FSKPRE
