ID   C25AA_BACTJ             Reviewed;         675 AA.
AC   O87906;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Pesticidal crystal protein Cry25Aa;
DE   AltName: Full=76 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXXVA(a);
DE   AltName: Full=Insecticidal protein Jeg74;
GN   Name=cry25Aa; Synonyms=cryXXVA(a);
OS   Bacillus thuringiensis subsp. jegathesan.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=56955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kawalek M.D., Gill S.S.;
RT   "Isolation and characterization of insecticidal genes from Bacillus
RT   thuringiensis subsp. jegathesan.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; U88189; AAC61892.1; -; Genomic_DNA.
DR   AlphaFoldDB; O87906; -.
DR   SMR; O87906; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..675
FT                   /note="Pesticidal crystal protein Cry25Aa"
FT                   /id="PRO_0000174100"
SQ   SEQUENCE   675 AA;  75643 MW;  509CC9F1F7501459 CRC64;
     MNPYQNKSEC EILNAPLNNI NMPNRYPFAN DPNAVMKNGN YKDWLNECDG ITPSIFGTLG
     VLASIVISTI NLATSPSIGD AFALVSSIGE YWPETKTSFP LSVADVNRLI REALDQNAIN
     RATGKFNGLM DTYNTVYLKN LQDWYDTRIP ANPQGDSQLR EAARRSLEEI ERDFRKALAG
     EFAEAGSQIV LLPIYAQAAN IHLLILKDAM QFRTDLGLIR PVGVPITTSA EDPFESEFLL
     RIKKYTDHCI SYYDDGLAKI RSRGSDGETW WEFNKFRREM TLTVLDLVAL YPTHNIKLYP
     IPTQTELSRV VYTDPVGCFG NRKSDIFSRL NFDYLENRLT RPREPFNYLN SVQLFASTVS
     NSNNGEVLRG NLNKIMFEGG WTASRSGDGV TTGTPFSTMD WSYGWGYPRK HYAEITSRSQ
     ALPGLNNSIH VIVGIDSFRA IGPGGQGDHT FSLPGGDMYD CGKVQINPLE DYRNSDHWIS
     DMMTINQSVQ LASNPTQTFA FSALSLGWHH SSAGNRNVYV YDKITQIPAT KTVREHPMIK
     GPGFTGGDLA DLSSNSDILQ YDLRSDYDDR LTEDVPFRIR IRCASIGVST ISVDNWGSSS
     PQVTVASTAA SLDTLKYESF QYVSIPGNYY FDSAPRIRLL RQPGRLLVDR IEIIPVNFFP
     LSEQENKSVD SLFIN