TRUB_CAMJE
ID TRUB_CAMJE Reviewed; 272 AA.
AC Q9PNJ2; Q0P9F2;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=Cj1102;
OS Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS 11168).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Campylobacteraceae; Campylobacter.
OX NCBI_TaxID=192222;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700819 / NCTC 11168;
RX PubMed=10688204; DOI=10.1038/35001088;
RA Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA Barrell B.G.;
RT "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT reveals hypervariable sequences.";
RL Nature 403:665-668(2000).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR EMBL; AL111168; CAL35219.1; -; Genomic_DNA.
DR PIR; A81314; A81314.
DR RefSeq; WP_010891909.1; NC_002163.1.
DR RefSeq; YP_002344495.1; NC_002163.1.
DR AlphaFoldDB; Q9PNJ2; -.
DR SMR; Q9PNJ2; -.
DR IntAct; Q9PNJ2; 7.
DR STRING; 192222.Cj1102; -.
DR PaxDb; Q9PNJ2; -.
DR PRIDE; Q9PNJ2; -.
DR EnsemblBacteria; CAL35219; CAL35219; Cj1102.
DR GeneID; 905393; -.
DR KEGG; cje:Cj1102; -.
DR PATRIC; fig|192222.6.peg.1084; -.
DR eggNOG; COG0130; Bacteria.
DR HOGENOM; CLU_032087_2_0_7; -.
DR OMA; ELQFIRW; -.
DR Proteomes; UP000000799; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01080; TruB_bact; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..272
FT /note="tRNA pseudouridine synthase B"
FT /id="PRO_0000121811"
FT ACT_SITE 38
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ SEQUENCE 272 AA; 31419 MW; 60A57C3ACE528684 CRC64;
MNKIFAAFKP RGLSSNAFLS TLKKKYKNKK AGYSGTLDPF AKGVLIVAFG QYTKLFRFLK
KTPKTYKATL WLGVYSLSLD DQNIKEIKNI KEFDLPNLQQ IIDQMQGIIS YTPPQFSAKR
INGTRAYELA KKGIEVNLKP CQMEVFDCKI LSYNHPFLNI EITVSEGAYI RSYCELFARK
LGINATLSSL ERIKEGKFVY NNEKSLNVLK YINLKPNFIK DLNKLENGAK IFVEELEFHD
KGDYYIETEK YFSIINIKEN TVKYLLNKVE KC
