ID   C25L2_DANRE             Reviewed;         279 AA.
AC   A8WGT1;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Cholesterol 25-hydroxylase-like protein 2 {ECO:0000312|ZFIN:ZDB-GENE-080204-82};
DE            EC=1.14.99.-;
GN   Name=ch25hl2 {ECO:0000312|ZFIN:ZDB-GENE-080204-82}; ORFNames=zgc:175201;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1] {ECO:0000312|EMBL:AAI54838.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo {ECO:0000312|EMBL:AAI54838.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May catalyze the formation of 25-hydroxycholesterol from
CC       cholesterol. {ECO:0000250|UniProtKB:Q9Z0F5}.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000250|UniProtKB:Q9Z0F5};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q9Z0F5}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q9Z0F5}.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000255}.
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DR   EMBL; BC154837; AAI54838.1; -; mRNA.
DR   RefSeq; NP_001108042.1; NM_001114570.1.
DR   AlphaFoldDB; A8WGT1; -.
DR   STRING; 7955.ENSDARP00000056559; -.
DR   PaxDb; A8WGT1; -.
DR   Ensembl; ENSDART00000056560; ENSDARP00000056559; ENSDARG00000038728.
DR   GeneID; 100136851; -.
DR   KEGG; dre:100136851; -.
DR   CTD; 100136851; -.
DR   ZFIN; ZDB-GENE-080204-82; ch25hl2.
DR   eggNOG; KOG0873; Eukaryota.
DR   GeneTree; ENSGT00940000163793; -.
DR   HOGENOM; CLU_047036_5_1_1; -.
DR   InParanoid; A8WGT1; -.
DR   OMA; TTWGFMV; -.
DR   OrthoDB; 1493916at2759; -.
DR   PhylomeDB; A8WGT1; -.
DR   TreeFam; TF314256; -.
DR   PRO; PR:A8WGT1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 21.
DR   Bgee; ENSDARG00000038728; Expressed in granulocyte and 9 other tissues.
DR   ExpressionAtlas; A8WGT1; baseline.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0000254; F:C-4 methylsterol oxidase activity; IBA:GO_Central.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0008395; F:steroid hydroxylase activity; IBA:GO_Central.
DR   GO; GO:0008203; P:cholesterol metabolic process; IBA:GO_Central.
DR   GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Iron; Lipid biosynthesis;
KW   Lipid metabolism; Membrane; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Steroid biosynthesis; Steroid metabolism;
KW   Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix.
FT   CHAIN           1..279
FT                   /note="Cholesterol 25-hydroxylase-like protein 2"
FT                   /id="PRO_0000397926"
FT   TRANSMEM        36..56
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /evidence="ECO:0000255"
FT   DOMAIN          128..262
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
FT   MOTIF           141..145
FT                   /note="Histidine box-1"
FT                   /evidence="ECO:0000255"
FT   MOTIF           156..160
FT                   /note="Histidine box-2"
FT                   /evidence="ECO:0000255"
FT   MOTIF           237..243
FT                   /note="Histidine box-3"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        6
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        13
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   279 AA;  32605 MW;  8EF436CE01F63A49 CRC64;
     MLDISNTTWD LSNQSVLQPL WDYLQQNHES SLRSPLFPVI LSVSMYLVLV FFYTVLDLLA
     PTWPSIRRYQ IHQDRTVTWS NIGSTLALTT YNHLLYIFPA AVAQWLWRPP IPLPREAPTL
     TAFLLGIVGC TVVFDFQYYL WHLLHHRVGW LYRTFHALHH QYRQTFSLVT QYLSAWELFS
     VGFWTTVDPL LLQCHCLTAW AFMLFNIWVS TEDHCGYDFP WAMHRLVPFG LWGGALRHDA
     HHQLPGTNFA PFFAHWDWLG GTATMPAPVK TKKRDDKEA