ID   C27AA_BACUH             Reviewed;         826 AA.
AC   Q9S597;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Pesticidal crystal protein Cry27Aa;
DE   AltName: Full=94 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXXVIIA(a);
GN   Name=cry27Aa; Synonyms=cryXXVIIA(a);
OS   Bacillus thuringiensis subsp. higo.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=132266;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Saitoh H.;
RT   "94kDa mosquitocidal toxin from serovar higo.";
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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DR   EMBL; AB023293; BAA82796.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9S597; -.
DR   SMR; Q9S597; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR038979; Pest_crys.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   PANTHER; PTHR37003; PTHR37003; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..826
FT                   /note="Pesticidal crystal protein Cry27Aa"
FT                   /id="PRO_0000174102"
FT   REGION          717..743
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   826 AA;  94435 MW;  DFD5313C5B6023E2 CRC64;
     MNPYQNKNEY EILDAKRNNC HMSNGYPRHP LANDPQMYLR NAHYKDWLSM CNKNNPVGLI
     PPESFEWTWL NGTVAALTIV SVIAGILVTA PVSVTAGLIT VLGAGAALLA GITPLIWPAT
     TDNTFNKITD ATEVLLNKEI SEFVRKTANT KIDSLQQLIY YYQNALENWK KNPNDSAARN
     TVSTRFQIVN AFFVEAMPAL SMPGYEVVQL GAYAQAANLH LILLREGIAY ADQWNLARDP
     MHAAGDLHYK EFLDYRNQYI NHCSTWYNEG QNEANLKNNG LVYQRTMTLF VLDLIAMFST
     YDPRLYTMPI KTEILTRTIY TDGVNRNEPK SIHNPGLFRR LEQMKLHIYE YQGAQFLSGH
     QNIFRSMNYN HPLIYGPVQG YSSSNINKIT TINLGDYDKI YSINTESRNR LVQGSTTFDK
     INFYGAFNEN WLFSVYNQNG PIIKHSNIPG IDAPSTGLNY SNYTHYLSNC IFQSNRNGGS
     APDYNTQSYV FGWNHYTIDP TGNYVTDAFE VNKNLPESRY VPQISQVPAV KASDIFNPGR
     VVNAKVESGP YFTGGDVIVS KAQLDGSGLA RTLITFPIIP KRYRASGFRV RMYYAANHTG
     QVSYGVANIN TTGYANFQKT FDGWEYFRAR HEHFKYIEFD TTFSLRNSGQ LEEHLLHIYY
     PNTTKISGDQ LLIIDKIEFI PVGIPLNQTS EGYNTYDQNT NSYNQNYNNY NQNMDTTYQP
     NYDNYKQNSS GMYDNPYNQN PKDSYNQNYT DTYDSGYNNS QNVGSNYNQE YNTYNQDTEN
     MYNQSYNNYN SDNNNYNQNS DCMCSPGYNG NYECRCNQRA NGNYPK