ID   C28AA_BACTF             Reviewed;        1109 AA.
AC   Q9X682;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Pesticidal crystal protein Cry28Aa;
DE   AltName: Full=126 kDa crystal protein;
DE   AltName: Full=Crystaline entomocidal protoxin;
DE   AltName: Full=Insecticidal delta-endotoxin CryXXVIIIA(a);
GN   Name=cry28Aa; Synonyms=cryXXVIIIA(a);
OS   Bacillus thuringiensis subsp. finitimus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=29337;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=VKPM B-1161;
RX   PubMed=10403372; DOI=10.1016/s0014-5793(99)00650-x;
RA   Wojciechowska J.A., Lewitin E., Revina L.P., Zalunin I.A.,
RA   Chestukhina G.G.;
RT   "Two novel delta-endotoxin gene families cry26 and cry28 from Bacillus
RT   thuringiensis ssp. finitimus.";
RL   FEBS Lett. 453:46-48(1999).
CC   -!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
CC       epithelial cells of insects.
CC   -!- DEVELOPMENTAL STAGE: The crystal protein is produced during sporulation
CC       and is accumulated both as an inclusion and as part of the spore coat.
CC   -!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
CC       terminus.
CC   -!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF132928; AAD24189.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9X682; -.
DR   SMR; Q9X682; -.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0001897; P:cytolysis by symbiont of host cells; IEA:InterPro.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.190.10; -; 1.
DR   Gene3D; 2.100.10.10; -; 1.
DR   InterPro; IPR041587; Cry_V.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005638; Pest_crys_C.
DR   InterPro; IPR005639; Pest_crys_N.
DR   InterPro; IPR036716; Pest_crys_N_sf.
DR   InterPro; IPR001178; Pest_cryst_cen_dom.
DR   InterPro; IPR036399; Pest_cryst_cen_dom_sf.
DR   Pfam; PF17997; Cry1Ac_D5; 1.
DR   Pfam; PF03944; Endotoxin_C; 1.
DR   Pfam; PF00555; Endotoxin_M; 1.
DR   Pfam; PF03945; Endotoxin_N; 1.
DR   SUPFAM; SSF49785; SSF49785; 2.
DR   SUPFAM; SSF51096; SSF51096; 1.
DR   SUPFAM; SSF56849; SSF56849; 1.
PE   2: Evidence at transcript level;
KW   Sporulation; Toxin; Virulence.
FT   CHAIN           1..1109
FT                   /note="Pesticidal crystal protein Cry28Aa"
FT                   /id="PRO_0000174103"
SQ   SEQUENCE   1109 AA;  125714 MW;  10C80705508F5CDA CRC64;
     MAQTYYKIGV QSTEVNSESI FFNPEVDSSD TVAVVSAGIV VVGTILTAFA SFVNPGVVLI
     SFGTLAPVLW PDPEEDPKKI WSQFMKHGED LLNQTISTAV KEIALAHLNG FKDVLTYYER
     AFNDWKRNPS ANTARLVSQR FENAHFNFVS NMPQLQLPTY DTLLLSCYTE AANLHLNLLH
     QGVQFADQWN ADQPHSPMLK SSGTYYDELL VYIEKYINYC TKTYHKGLNH LKESEKITWD
     AYNTYRREMT LIVLDLVATF PFYDIRRFPR GVELELTREV YTSLDHLTRP PGLFTWLSDI
     ELYTESVAEG DYLSGIRESK YYTGNQFFTM KNIYGNTNRL SKQLITLLPG EFMTHLSINR
     PFQTIAGINK LYSLIQKIVF TTFKNDNEYQ KNFNVNNQNE PQETTNYPND YGGSNSQKFK
     HNLSHFPLII HKLEFAEYFH SIFALGWTHN SVNSQNLISE SVSTQIPLVK AYEVTNNSVI
     RGPGFTGGDL IELRDKCSIK CKASSLKKYA ISLFYAANNA IAVSIDVGDS GAGVLLQPTF
     SRKGNNNFTI QDLNYKDFQY HTLLVDIELP ESEEIHIHLK REDDYEEGVI LLIDKLEFKP
     IDENYTNEMN LEKAKKAVNV LFINATNALK MDVTDYHIDQ VANLVECISD DLYAKEKIKF
     TPCIKFAKQL SQARNLLSDP NFNNLNAENS WTANTGVTII EGDPLYKGRA IQLSAARDEN
     FPTYLYQKID ESLLKPYTRY QLRGFVEGSQ DLELDLVRYG ATDIVMNVPG DLEILSYSAP
     INPCEEIETR LDTTCGALDR CKQSNYVNSA ADVRPDQVNG DPHAFSFHID TGTTDNNRNL
     GIWIIFKIAT PDGYATFGNL ELIELGPLSG EALAQVQRKE QKWGKNTTQK REEAAKLYAA
     AKQTINQLFA DSQGTKLRFD TEFSNILSAD KLVYKIRDVY SEVLSVIPGL NYDLFMELEN
     RIQNAIDLYD ARNTVTNGEF RNGLANWMAS SNTEVRQIQA HPCWYSLGWN AQVAQSLNVK
     PDHGYVLRVT AKKEGIGNGY VTILDCANHI DTLTFSSCDS GFTTSSNELA AYVTKTLEIF
     PDTDQIRIEI GETRSTFYVE SVDLIRMED