ID   TRUB_HALSA              Reviewed;         301 AA.
AC   Q9HPA4;
DT   04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 120.
DE   RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01081};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01081};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01081};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01081}; Synonyms=sus;
GN   OrderedLocusNames=VNG_1729G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01081}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01081};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01081}.
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DR   EMBL; AE004437; AAG19966.1; -; Genomic_DNA.
DR   PIR; B84325; B84325.
DR   RefSeq; WP_012289369.1; NC_002607.1.
DR   AlphaFoldDB; Q9HPA4; -.
DR   SMR; Q9HPA4; -.
DR   STRING; 64091.VNG_1729G; -.
DR   PaxDb; Q9HPA4; -.
DR   EnsemblBacteria; AAG19966; AAG19966; VNG_1729G.
DR   GeneID; 5953647; -.
DR   KEGG; hal:VNG_1729G; -.
DR   PATRIC; fig|64091.14.peg.1319; -.
DR   HOGENOM; CLU_032087_3_0_2; -.
DR   InParanoid; Q9HPA4; -.
DR   OMA; GPFKEDE; -.
DR   OrthoDB; 36028at2157; -.
DR   PhylomeDB; Q9HPA4; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000495; P:box H/ACA RNA 3'-end processing; IBA:GO_Central.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031118; P:rRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031120; P:snRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01081; TruB_arch; 1.
DR   InterPro; IPR012960; Dyskerin-like.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR   InterPro; IPR026326; TruB_arch.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR23127; PTHR23127; 1.
DR   Pfam; PF08068; DKCLD; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 2.
DR   SMART; SM01136; DKCLD; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00425; CBF5; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..301
FT                   /note="Probable tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121957"
FT   DOMAIN          227..301
FT                   /note="PUA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT   ACT_SITE        54
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
SQ   SEQUENCE   301 AA;  31825 MW;  063C79388CFF1A0C CRC64;
     MGIRPPPGER SPAAVLSFGV VNLDKPPGPS AHQVSAWIRD LVGVEKAAHA GTLDPKVTGC
     LPVLTGTATR IAPALLEGFK EYVAVLELHD DPPRILPDVI EAFTGEIYQK PPKKSAVARR
     LRTRTVYDLD VLDVDGRQVL LRIRCESGTY IRKLCHDIGR ALGTNAHMGH LRRSATTPFD
     DTDLVTLHDL ADAVAWLRDT DDTEPPDAPA DALRAAVQPA ERALTHLPRL TIADSAAHEV
     ATGAPVYAPG VIDTTALPTP PADGALVACY TAGGTAVCLG RLVGDPDADA GVVVALERVL
     V