TRUB_LACAC
ID TRUB_LACAC Reviewed; 297 AA.
AC Q5FJN8;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=LBA1253;
OS Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=272621;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA Hamrick A., Cano R., Klaenhammer T.R.;
RT "Complete genome sequence of the probiotic lactic acid bacterium
RT Lactobacillus acidophilus NCFM.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR EMBL; CP000033; AAV43086.1; -; Genomic_DNA.
DR RefSeq; WP_003547805.1; NC_006814.3.
DR RefSeq; YP_194117.1; NC_006814.3.
DR AlphaFoldDB; Q5FJN8; -.
DR SMR; Q5FJN8; -.
DR STRING; 272621.LBA1253; -.
DR PRIDE; Q5FJN8; -.
DR EnsemblBacteria; AAV43086; AAV43086; LBA1253.
DR GeneID; 56942844; -.
DR KEGG; lac:LBA1253; -.
DR PATRIC; fig|272621.13.peg.1188; -.
DR eggNOG; COG0130; Bacteria.
DR HOGENOM; CLU_032087_0_1_9; -.
DR OMA; ELQFIRW; -.
DR BioCyc; LACI272621:G1G49-1236-MON; -.
DR Proteomes; UP000006381; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR HAMAP; MF_01080; TruB_bact; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR InterPro; IPR032819; TruB_C.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..297
FT /note="tRNA pseudouridine synthase B"
FT /id="PRO_0000121847"
FT ACT_SITE 39
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ SEQUENCE 297 AA; 33621 MW; FD5D5B88080E5769 CRC64;
MLNGIIVIDK DKGMTSADVV YHLRRALHIR KIGHAGTLDP EVTGVLPIAI GQATKLIELM
HTRPKKYQGT GLFGYATDSY DIDGKVLKEK RLVMPFTVEE IQRGMNTFKG KIEQVPPIYS
AVKVNGKHLY EYAREGIKVE RPKRQVEIFQ YDLLNDPSFD KEKGQESFDF EITCSKGTYV
RSLVNDLGEK LDEPAVMTYL RRVASSGFDI SQAVKLSEIE ANPEKASELI QPIDAFFKDY
ETIDLPEGKW LKVKNGAGIS LETNAKKVAL RYNEKVKAIY EKKGKIYRPS LMLLQNE
