ID   TRUB_MESFL              Reviewed;         286 AA.
AC   Q6F1I4;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=Mfl282;
OS   Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1)
OS   (Acholeplasma florum).
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae;
OC   Mesoplasma.
OX   NCBI_TaxID=265311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33453 / NBRC 100688 / NCTC 11704 / L1;
RA   Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S.,
RA   Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR   EMBL; AE017263; AAT75639.1; -; Genomic_DNA.
DR   RefSeq; WP_011183179.1; NC_006055.1.
DR   RefSeq; YP_053523.1; NC_006055.1.
DR   AlphaFoldDB; Q6F1I4; -.
DR   SMR; Q6F1I4; -.
DR   STRING; 265311.Mfl282; -.
DR   EnsemblBacteria; AAT75639; AAT75639; Mfl282.
DR   GeneID; 2897965; -.
DR   KEGG; mfl:Mfl282; -.
DR   PATRIC; fig|265311.5.peg.282; -.
DR   eggNOG; COG0130; Bacteria.
DR   HOGENOM; CLU_032087_0_2_14; -.
DR   OMA; ELQFIRW; -.
DR   Proteomes; UP000006647; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..286
FT                   /note="tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121861"
FT   ACT_SITE        40
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ   SEQUENCE   286 AA;  32451 MW;  C17F2040CEB1ED7C CRC64;
     MNKSGIIILN KPKGLTTNHL IQKLKRKLNV KKIGHAGTLD PLATGVVICL INNGTKLSDY
     FLNENKAYEV TMKLFQATDT YDSDGSIIEE QEPFKIEQEQ VEKVIAKFNG LAYEQEPPMY
     SAIKIDGKKL YEYARENQVV KVNKRLIKIN SLSLDKYENN EISMTVYCSK GTYIRSLIVD
     IAKELNTIAH VTSLNRIESG NFIIKNSVNI ENCEESNLIK MFDAIKMADY EIVELDDTLN
     VEHGKKIEVI AEKNIVFISN KSKELIACYE RENGNVFKCK RGGLNI