TRUB_METJA
ID TRUB_METJA Reviewed; 336 AA.
AC Q57612;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01081}; OrderedLocusNames=MJ0148;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01081};
CC -!- INTERACTION:
CC Q57612; P81303: nop10; NbExp=5; IntAct=EBI-9026474, EBI-9026465;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01081}.
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DR EMBL; L77117; AAB98132.1; -; Genomic_DNA.
DR PIR; E64318; E64318.
DR RefSeq; WP_010869643.1; NC_000909.1.
DR PDB; 2APO; X-ray; 1.95 A; A=1-336.
DR PDBsum; 2APO; -.
DR AlphaFoldDB; Q57612; -.
DR SMR; Q57612; -.
DR DIP; DIP-60499N; -.
DR IntAct; Q57612; 2.
DR STRING; 243232.MJ_0148; -.
DR EnsemblBacteria; AAB98132; AAB98132; MJ_0148.
DR GeneID; 1450992; -.
DR KEGG; mja:MJ_0148; -.
DR eggNOG; arCOG00987; Archaea.
DR HOGENOM; CLU_032087_3_0_2; -.
DR InParanoid; Q57612; -.
DR OMA; GPFKEDE; -.
DR OrthoDB; 36028at2157; -.
DR PhylomeDB; Q57612; -.
DR EvolutionaryTrace; Q57612; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000495; P:box H/ACA RNA 3'-end processing; IBA:GO_Central.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031118; P:rRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031120; P:snRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.130.10; -; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR23127; PTHR23127; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00425; CBF5; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..336
FT /note="Probable tRNA pseudouridine synthase B"
FT /id="PRO_0000121959"
FT DOMAIN 248..323
FT /note="PUA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT ACT_SITE 81
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT STRAND 18..22
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 34..36
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 39..44
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 46..52
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 54..56
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 58..68
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 74..78
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 85..92
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 93..101
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 107..117
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 121..131
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 132..136
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 151..163
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 166..173
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 179..189
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 194..204
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 209..211
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 215..226
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 232..237
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 238..240
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 241..245
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 248..253
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 255..257
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 258..262
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 269..271
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 272..276
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 284..288
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 294..302
FT /evidence="ECO:0007829|PDB:2APO"
FT HELIX 304..309
FT /evidence="ECO:0007829|PDB:2APO"
FT STRAND 311..321
FT /evidence="ECO:0007829|PDB:2APO"
SQ SEQUENCE 336 AA; 38483 MW; F29E3EC979A5A89A CRC64;
MILLEKTQEK KINDKEELIV KEEVETNWDY GCNPYERKIE DLIKYGVVVV DKPRGPTSHE
VSTWVKKILN LDKAGHGGTL DPKVTGVLPV ALERATKTIP MWHIPPKEYV CLMHLHRDAS
EEDILRVFKE FTGRIYQRPP LKAAVKRRLR IRKIHELELL DKDGKDVLFR VKCQSGTYIR
KLCEDIGEAL GTSAHMQELR RTKSGCFEEK DAVYLQDLLD AYVFWKEDGD EEELRRVIKP
MEYGLRHLKK VVVKDSAVDA ICHGADVYVR GIAKLSKGIG KGETVLVETL KGEAVAVGKA
LMNTKEILNA DKGVAVDVER VYMDRGTYPR MWKRKK
