C298B_XENLA
ID C298B_XENLA Reviewed; 273 AA.
AC A0A1L8H579;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 1.
DT 25-MAY-2022, entry version 16.
DE RecName: Full=Cilia- and flagella-associated protein 298-B {ECO:0000305};
DE AltName: Full=Protein kurly-B {ECO:0000305};
GN Name=cfap298-b; Synonyms=kur-b {ECO:0000303|PubMed:26904945};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355 {ECO:0000312|Proteomes:UP000186698};
RN [1] {ECO:0000312|Proteomes:UP000186698}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J {ECO:0000312|Proteomes:UP000186698};
RX PubMed=27762356; DOI=10.1038/nature19840;
RA Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL Nature 538:336-343(2016).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=26904945; DOI=10.1016/j.celrep.2016.01.069;
RA Jaffe K.M., Grimes D.T., Schottenfeld-Roames J., Werner M.E., Ku T.S.,
RA Kim S.K., Pelliccia J.L., Morante N.F., Mitchell B.J., Burdine R.D.;
RT "c21orf59/kurly controls both cilia motility and polarization.";
RL Cell Rep. 14:1841-1849(2016).
CC -!- FUNCTION: Plays a role in motile cilium function, possibly by acting on
CC outer dynein arm assembly (PubMed:26904945). Seems to be important for
CC initiation rather than maintenance of cilium motility (By similarity).
CC Required for correct positioning of the cilium at the apical cell
CC surface, suggesting an additional role in the planar cell polarity
CC (PCP) pathway (PubMed:26904945). May suppress canonical Wnt signaling
CC activity (PubMed:26904945). {ECO:0000250|UniProtKB:Q6DRC3,
CC ECO:0000269|PubMed:26904945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26904945}.
CC Cytoplasm, cytoskeleton, cilium basal body
CC {ECO:0000269|PubMed:26904945}. Note=Appears in cytoplasmic puncta,
CC compatible with a centrosomal localization (By similarity).
CC {ECO:0000250|UniProtKB:Q5U3Z0}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein results in
CC loss of cilia-driven fluid flow along the anterior-posterior axis.
CC Multiciliated cells show loss of coordinated cilium polarity with many
CC cilia pointing towards the anterior instead of the posterior. The
CC planar cell polarity (PCP) component prickle2 is uniformly distributed
CC around the cell margin, instead of being asymmetrically localized to
CC the posterior of the cell. {ECO:0000269|PubMed:26904945}.
CC -!- SIMILARITY: Belongs to the CFAP298 family. {ECO:0000305}.
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DR EMBL; CM004469; OCT91244.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1L8H579; -.
DR STRING; 8355.A0A1L8H579; -.
DR OMA; HAPNDSM; -.
DR Proteomes; UP000186698; Genome assembly.
DR GO; GO:0005929; C:cilium; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0003352; P:regulation of cilium movement; IEA:InterPro.
DR InterPro; IPR021298; CFAP298.
DR PANTHER; PTHR13238; PTHR13238; 1.
DR Pfam; PF11069; CFAP298; 1.
PE 3: Inferred from homology;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Reference proteome.
FT CHAIN 1..273
FT /note="Cilia- and flagella-associated protein 298-B"
FT /id="PRO_0000441860"
SQ SEQUENCE 273 AA; 31399 MW; 9F1F36A0CC5D157E CRC64;
MVRLHIKRGD ESQFLFDTSV IVPVEALVKQ ITAIYNGILK IDRICSEMAE LAEHDLKLKD
EWEERCVPSG GSVFKKDEIG RRNGHAPNDS MKKVLQKTME EAKALISKKQ AEANVCVTLE
MVKEATDQLR GAVMIVYPMG LPPHDPIRME FENNEDLSGT HAGQLVIQEP ESQLWWAGKE
LQRKQKLSDY VGKNEKTKII VKIQKRGQGA PGREPVISQE EQKNLMMHYY RRQEELKKLE
VDEDISYLNA EWADSNSLKR QFQGVNDIKW KPR