ID   TRUB_MYCGA              Reviewed;         214 AA.
AC   P59879;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=tRNA pseudouridine synthase B;
DE            EC=5.4.99.25;
DE   AltName: Full=tRNA pseudouridine(55) synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=truB; OrderedLocusNames=MYCGA1190; ORFNames=MGA_0831;
OS   Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
OS   (Mycoplasmoides gallisepticum).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=710127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R(low / passage 15 / clone 2);
RX   PubMed=12949158; DOI=10.1099/mic.0.26427-0;
RA   Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F.,
RA   Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.;
RT   "The complete genome sequence of the avian pathogen Mycoplasma
RT   gallisepticum strain R(low).";
RL   Microbiology 149:2307-2316(2003).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE015450; AAP56469.2; -; Genomic_DNA.
DR   RefSeq; WP_011113348.1; NC_004829.2.
DR   AlphaFoldDB; P59879; -.
DR   SMR; P59879; -.
DR   GeneID; 57203397; -.
DR   KEGG; mga:MGA_0831; -.
DR   HOGENOM; CLU_032087_2_0_14; -.
DR   OrthoDB; 1166299at2; -.
DR   Proteomes; UP000001418; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..214
FT                   /note="tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121864"
FT   ACT_SITE        41
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   214 AA;  24718 MW;  617FE612E4A2C0B3 CRC64;
     MDFNNKIVII DKPKNISSAF CLNLFKKRFN IKKAGHNGTL DPLASGVLVV ATNKRTKELS
     QLNQDDKQYL VKLKFNTHTD SYDRLGKVIR TTNYVPEIKQ LNDYLTSLDN HSFYQLPPNF
     SALKVNGVRA YQLARKAVDF ELEKRPTTIY KAKLISYYDD YAEILLDVKK GFYVRSFVVD
     LASQFNTTAM MVDLVRTRSG QYSLKDVIDY QFNK