TRUB_MYCPU
ID TRUB_MYCPU Reviewed; 282 AA.
AC Q98Q19;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=MYPU_5500;
OS Mycoplasmopsis pulmonis (strain UAB CTIP) (Mycoplasma pulmonis).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasmopsis.
OX NCBI_TaxID=272635;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UAB CTIP;
RX PubMed=11353084; DOI=10.1093/nar/29.10.2145;
RA Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F.,
RA Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A.;
RT "The complete genome sequence of the murine respiratory pathogen Mycoplasma
RT pulmonis.";
RL Nucleic Acids Res. 29:2145-2153(2001).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR EMBL; AL445565; CAC13723.1; -; Genomic_DNA.
DR PIR; F90580; F90580.
DR RefSeq; WP_010925351.1; NC_002771.1.
DR AlphaFoldDB; Q98Q19; -.
DR SMR; Q98Q19; -.
DR STRING; 272635.MYPU_5500; -.
DR PRIDE; Q98Q19; -.
DR EnsemblBacteria; CAC13723; CAC13723; CAC13723.
DR KEGG; mpu:MYPU_5500; -.
DR eggNOG; COG0130; Bacteria.
DR HOGENOM; CLU_032087_0_2_14; -.
DR OMA; ELQFIRW; -.
DR OrthoDB; 1166299at2; -.
DR BioCyc; MPUL272635:G1GT6-558-MON; -.
DR Proteomes; UP000000528; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR HAMAP; MF_01080; TruB_bact; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..282
FT /note="tRNA pseudouridine synthase B"
FT /id="PRO_0000121870"
FT ACT_SITE 36
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ SEQUENCE 282 AA; 31961 MW; A598529F52B1EBEE CRC64;
MFYLIYKEKG ISSFKAIKDF AWQNNIKKIG HSGTLDPEAT GLLLLASDED TKLLDYVDKK
FKSYRATMIL GLQSQSFDSQ GKIINSSNLK VDNLTIEKTI KNFVGPFVQI PPIFSAKKIN
GKRAYEYARQ GSEISMKAQE VFVKSIEIEK IDFPKVIFKA KVSRGTYIRS LINQIGLELK
TYALMDDLER IELSGLSKND LGVVSDLDII DLEVLSLEKH EILTLAKGQK FSKDLADGKY
AFIYKNTKKI LGICKIESKI IAPIKIFNKK IEKSLKKDEK NE
