ID   TRUB_PSEAE              Reviewed;         304 AA.
AC   P72154; Q9HV57;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 2.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=tRNA pseudouridine synthase B;
DE            EC=5.4.99.25;
DE   AltName: Full=Osmoprotectant regulator of PLC;
DE            Short=ORP protein;
DE   AltName: Full=tRNA pseudouridine(55) synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=truB; Synonyms=orp; OrderedLocusNames=PA4742;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-139.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=9004219; DOI=10.1046/j.1365-2958.1997.1681542.x;
RA   Sage A.E., Vasil A.I., Vasil M.L.;
RT   "Molecular characterization of mutants affected in the osmoprotectant-
RT   dependent induction of phospholipase C in Pseudomonas aeruginosa PAO1.";
RL   Mol. Microbiol. 23:43-56(1997).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs (By similarity). Osmoprotectant
CC       regulator of PLC. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE004091; AAG08128.1; -; Genomic_DNA.
DR   EMBL; U54794; AAB48163.1; -; Genomic_DNA.
DR   PIR; E83052; E83052.
DR   RefSeq; NP_253430.1; NC_002516.2.
DR   RefSeq; WP_003100522.1; NZ_QZGE01000018.1.
DR   AlphaFoldDB; P72154; -.
DR   SMR; P72154; -.
DR   STRING; 287.DR97_2085; -.
DR   PaxDb; P72154; -.
DR   PRIDE; P72154; -.
DR   EnsemblBacteria; AAG08128; AAG08128; PA4742.
DR   GeneID; 881676; -.
DR   KEGG; pae:PA4742; -.
DR   PATRIC; fig|208964.12.peg.4968; -.
DR   PseudoCAP; PA4742; -.
DR   HOGENOM; CLU_032087_0_3_6; -.
DR   InParanoid; P72154; -.
DR   OMA; ELQFIRW; -.
DR   PhylomeDB; P72154; -.
DR   BioCyc; PAER208964:G1FZ6-4852-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0006400; P:tRNA modification; IBA:GO_Central.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF09157; TruB-C_2; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..304
FT                   /note="tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121888"
FT   ACT_SITE        48
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   304 AA;  33410 MW;  9C23F2016BDF8290 CRC64;
     MAQVKRIRRS ISGILVLDKP RGMSSNQALQ KVRWLLNAEK AGHTGSLDPL ATGVLPLCFG
     EATKFSQYLL DADKGYETVM RMGITTTTGD AEGELLAERD VTVGRDDLEQ ALPRFRGDIE
     QVPPMYSALK KDGQPLYKLA RAGEVVEREA RSVTITRLDL LSFEPPCATL AVSCSKGTYV
     RTLVEDLGQV LGCGAHVAAL RRTQAGPFAL AQAITLETLE RVHAEGGPEA LDQFLMPEDS
     GLLHWPVLQL SEHSAYYWLH GQPVRAPEAP KFGWLRVQDH TGRFIGIGEV TDDGRIAPRR
     LIRS