TRUB_PYRAB
ID TRUB_PYRAB Reviewed; 334 AA.
AC Q9V1A5; G8ZGN2;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01081}; OrderedLocusNames=PYRAB05220;
GN ORFNames=PAB0356;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01081};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01081}.
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DR EMBL; AJ248284; CAB49444.1; -; Genomic_DNA.
DR EMBL; HE613800; CCE69911.1; -; Genomic_DNA.
DR PIR; E75170; E75170.
DR RefSeq; WP_010867646.1; NC_000868.1.
DR PDB; 2AUS; X-ray; 2.10 A; A/C=1-334.
DR PDBsum; 2AUS; -.
DR AlphaFoldDB; Q9V1A5; -.
DR SMR; Q9V1A5; -.
DR STRING; 272844.PAB0356; -.
DR EnsemblBacteria; CAB49444; CAB49444; PAB0356.
DR GeneID; 1495424; -.
DR KEGG; pab:PAB0356; -.
DR PATRIC; fig|272844.11.peg.557; -.
DR eggNOG; arCOG00987; Archaea.
DR HOGENOM; CLU_032087_3_0_2; -.
DR OMA; GPFKEDE; -.
DR OrthoDB; 36028at2157; -.
DR PhylomeDB; Q9V1A5; -.
DR BRENDA; 5.4.99.25; 5242.
DR EvolutionaryTrace; Q9V1A5; -.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.130.10; -; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR23127; PTHR23127; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 2.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00425; CBF5; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; tRNA processing.
FT CHAIN 1..334
FT /note="Probable tRNA pseudouridine synthase B"
FT /id="PRO_0000121966"
FT DOMAIN 250..325
FT /note="PUA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 40..45
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 47..53
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 59..69
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 86..93
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 94..103
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 108..118
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 122..131
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 152..164
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 167..174
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 180..191
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 195..205
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 208..211
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 217..228
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 234..239
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 243..247
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 252..255
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 257..264
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 271..273
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 274..278
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 286..291
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 296..304
FT /evidence="ECO:0007829|PDB:2AUS"
FT HELIX 306..311
FT /evidence="ECO:0007829|PDB:2AUS"
FT STRAND 313..323
FT /evidence="ECO:0007829|PDB:2AUS"
SQ SEQUENCE 334 AA; 37728 MW; 68B91D2D0BDC0262 CRC64;
MARDEVRRIL PADIKREVIV KDDKAETNPK WGFPPDKRPI ELHIQYGVIN LDKPPGPTSH
EVVAWIKRIL NLEKAGHGGT LDPKVSGVLP VALERATRVV QALLPAGKEY VALMHLHGDV
PEDKIRAVMK EFEGEIIQRP PLRSAVKRRL RTRKVYYIEI LEIDGRDVLF RVGVEAGTYI
RSLIHHIGLA LGVGAHMAEL RRTRSGPFKE DETLVTLHDL VDYYHFWKED GIEEYIRKAI
QPMEKAVEHL PKIWIKDSAV AAVAHGANLT VPGIVKLNAG IKKGDLVAIM TLKDELVALG
KAMMSTQEMI ERSKGIAVDV EKVFMPRDWY PKLW
