TRUB_PYRFU
ID TRUB_PYRFU Reviewed; 340 AA.
AC Q7LWY0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01081}; OrderedLocusNames=PF1785;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01081};
CC -!- INTERACTION:
CC Q7LWY0; Q8U1R4: nop10; NbExp=6; IntAct=EBI-9025178, EBI-9025188;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01081}.
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DR EMBL; AE009950; AAL81909.1; -; Genomic_DNA.
DR RefSeq; WP_011012926.1; NZ_CP023154.1.
DR PDB; 2EY4; X-ray; 2.11 A; A/B=1-333.
DR PDB; 2HVY; X-ray; 2.30 A; A=1-340.
DR PDB; 2RFK; X-ray; 2.87 A; A=5-338.
DR PDB; 3HAX; X-ray; 2.11 A; A=1-340.
DR PDB; 3HAY; X-ray; 4.99 A; A=1-340.
DR PDB; 3HJW; X-ray; 2.35 A; A=8-334.
DR PDB; 3HJY; X-ray; 3.65 A; A=8-334.
DR PDB; 3LWO; X-ray; 2.86 A; A=1-340.
DR PDB; 3LWP; X-ray; 2.50 A; A=1-340.
DR PDB; 3LWQ; X-ray; 2.68 A; A=1-340.
DR PDB; 3LWR; X-ray; 2.20 A; A=1-340.
DR PDB; 3LWV; X-ray; 2.50 A; A=1-340.
DR PDB; 3MQK; X-ray; 2.80 A; A=8-335.
DR PDBsum; 2EY4; -.
DR PDBsum; 2HVY; -.
DR PDBsum; 2RFK; -.
DR PDBsum; 3HAX; -.
DR PDBsum; 3HAY; -.
DR PDBsum; 3HJW; -.
DR PDBsum; 3HJY; -.
DR PDBsum; 3LWO; -.
DR PDBsum; 3LWP; -.
DR PDBsum; 3LWQ; -.
DR PDBsum; 3LWR; -.
DR PDBsum; 3LWV; -.
DR PDBsum; 3MQK; -.
DR AlphaFoldDB; Q7LWY0; -.
DR SMR; Q7LWY0; -.
DR DIP; DIP-48526N; -.
DR IntAct; Q7LWY0; 1.
DR STRING; 186497.PF1785; -.
DR PRIDE; Q7LWY0; -.
DR EnsemblBacteria; AAL81909; AAL81909; PF1785.
DR GeneID; 41713603; -.
DR KEGG; pfu:PF1785; -.
DR PATRIC; fig|186497.12.peg.1856; -.
DR eggNOG; arCOG00987; Archaea.
DR HOGENOM; CLU_032087_3_0_2; -.
DR OMA; GPFKEDE; -.
DR OrthoDB; 36028at2157; -.
DR PhylomeDB; Q7LWY0; -.
DR EvolutionaryTrace; Q7LWY0; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.130.10; -; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR23127; PTHR23127; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 2.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00425; CBF5; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..340
FT /note="Probable tRNA pseudouridine synthase B"
FT /id="PRO_0000121968"
FT DOMAIN 250..325
FT /note="PUA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT HELIX 11..13
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 40..45
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 47..53
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 59..69
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 86..93
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 94..99
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 100..103
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:2RFK"
FT STRAND 108..118
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 122..130
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 151..164
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 167..174
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 180..190
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 191..193
FT /evidence="ECO:0007829|PDB:3HAX"
FT STRAND 195..205
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 208..211
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 214..216
FT /evidence="ECO:0007829|PDB:3HJW"
FT HELIX 217..230
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 234..238
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 243..247
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 252..255
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 257..263
FT /evidence="ECO:0007829|PDB:2EY4"
FT TURN 264..266
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 271..273
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 274..278
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 286..291
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 296..304
FT /evidence="ECO:0007829|PDB:2EY4"
FT HELIX 306..311
FT /evidence="ECO:0007829|PDB:2EY4"
FT STRAND 314..323
FT /evidence="ECO:0007829|PDB:2EY4"
SQ SEQUENCE 340 AA; 38561 MW; 76CFE5095C5562FC CRC64;
MARDEVRRIL PADIKREVLI KDENAETNPD WGFPPEKRPI EMHIQFGVIN LDKPPGPTSH
EVVAWIKKIL NLEKAGHGGT LDPKVSGVLP VALEKATRVV QALLPAGKEY VALMHLHGDV
PEDKIIQVMK EFEGEIIQRP PLRSAVKRRL RTRKVYYIEV LEIEGRDVLF RVGVEAGTYI
RSLIHHIGLA LGVGAHMSEL RRTRSGPFKE DETLITLHDL VDYYYFWKED GIEEYFRKAI
QPMEKAVEHL PKVWIKDSAV AAVTHGADLA VPGIAKLHAG IKRGDLVAIM TLKDELVALG
KAMMTSQEML EKTKGIAVDV EKVFMPRDWY PKLWEKRDRS
