TRUB_RHOBA
ID TRUB_RHOBA Reviewed; 348 AA.
AC P59883;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2003, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=RB6411;
OS Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1).
OC Bacteria; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae;
OC Rhodopirellula.
OX NCBI_TaxID=243090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 10527 / NCIMB 13988 / SH1;
RX PubMed=12835416; DOI=10.1073/pnas.1431443100;
RA Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W.,
RA Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R.,
RA Reinhardt R.;
RT "Complete genome sequence of the marine planctomycete Pirellula sp. strain
RT 1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR EMBL; BX294144; CAD74785.1; -; Genomic_DNA.
DR RefSeq; NP_867239.1; NC_005027.1.
DR RefSeq; WP_011120904.1; NC_005027.1.
DR AlphaFoldDB; P59883; -.
DR SMR; P59883; -.
DR STRING; 243090.RB6411; -.
DR PRIDE; P59883; -.
DR EnsemblBacteria; CAD74785; CAD74785; RB6411.
DR KEGG; rba:RB6411; -.
DR PATRIC; fig|243090.15.peg.3098; -.
DR eggNOG; COG0130; Bacteria.
DR HOGENOM; CLU_032087_0_0_0; -.
DR InParanoid; P59883; -.
DR OrthoDB; 1166299at2; -.
DR Proteomes; UP000001025; Chromosome.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0006400; P:tRNA modification; IBA:GO_Central.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR HAMAP; MF_01080; TruB_bact; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..348
FT /note="tRNA pseudouridine synthase B"
FT /id="PRO_0000121892"
FT ACT_SITE 52
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ SEQUENCE 348 AA; 38421 MW; EB03F6A98697709D CRC64;
MDPNGEHSPL GFLPCYKPPG ATSRDLVNRA QRRLRGEFGL RKLKVGHTGT LDPLAEGLVL
LAIGSAARLT PWVLQHGKRY LADFRLGVSS ESGDLESELV TQTDVKLPTA AEIEQVLKDF
HGVVEQTPPA HSAIKVDGER AHKRARRGED FEMPKRRILI DSVKLISYEP PMMRLDVRCG
SGTYLRTLGM DVAAACGCAA VMTKLIRNEV GRFTLDDTLD CAFMFDDDDR EKMSSEPMVK
YLRPAIEGLT HMPAMNLDRQ QIGMLQAGIR ISGTPEAPSE PLPEAWIGCI DQVDTFDRNT
DVLDCIGVDR SDGSSGPWGE LVAILRPHGK LWHPLRVFPT TESITLRG
