ID   TRUB_RICCN              Reviewed;         345 AA.
AC   Q92HV5;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=tRNA pseudouridine synthase B;
DE            EC=5.4.99.25;
DE   AltName: Full=tRNA pseudouridine(55) synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=truB; OrderedLocusNames=RC0665;
OS   Rickettsia conorii (strain ATCC VR-613 / Malish 7).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=272944;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-613 / Malish 7;
RX   PubMed=11557893; DOI=10.1126/science.1061471;
RA   Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V.,
RA   Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.;
RT   "Mechanisms of evolution in Rickettsia conorii and R. prowazekii.";
RL   Science 293:2093-2098(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE006914; AAL03203.1; -; Genomic_DNA.
DR   PIR; A97783; A97783.
DR   RefSeq; WP_010977289.1; NC_003103.1.
DR   AlphaFoldDB; Q92HV5; -.
DR   SMR; Q92HV5; -.
DR   EnsemblBacteria; AAL03203; AAL03203; RC0665.
DR   KEGG; rco:RC0665; -.
DR   PATRIC; fig|272944.4.peg.758; -.
DR   HOGENOM; CLU_032087_0_3_5; -.
DR   OMA; IYELEMT; -.
DR   Proteomes; UP000000816; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR005728; Rickett_RPE.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR01045; RPE1; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..345
FT                   /note="tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121894"
FT   DOMAIN          261..309
FT                   /note="RPE1 insert"
FT   ACT_SITE        39
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   345 AA;  38747 MW;  D4AA33949F51AAB7 CRC64;
     MSNYWLNIYK PRGISSAQLV SIVKKILGKT KIGHAGTLDV EAEGILPFAV GEATKLIHLL
     VDARKTYIFT VKFGMQTNSG DCAGKVIATK DCIPSQEEAY AVCSKFIGNV TQIPPAFSAL
     KVNGVRAYKL AREGKKVELK PRNITIYDLK CLNFDEKNAT ATYYTECSKG TYIRTLAEDL
     ALSLQSLGFV IELRRTQVGI FKEENAIRIK SPDEITKNAL EENSIKIEAI LDDILVLDAT
     DSQAQQIKYG QKCLFNYEKD FRHLAKFAYR EEFKGNTERN TTAYTLVRED ASTGLTYKLP
     LEVEFGKMSV DLLWVRYKGT LLAIGSLNKS CFNSLRVFNL TQDFF