ID   TRUB_RICPR              Reviewed;         172 AA.
AC   Q9ZD46;
DT   04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Putative tRNA pseudouridine synthase B;
DE            EC=5.4.99.25;
DE   AltName: Full=tRNA pseudouridine(55) synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=truB; OrderedLocusNames=RP501;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. C-terminally truncated
CC       compared to other bacterial TruB. {ECO:0000305}.
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DR   EMBL; AJ235272; CAA14953.1; -; Genomic_DNA.
DR   PIR; G71653; G71653.
DR   RefSeq; NP_220877.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZD46; -.
DR   SMR; Q9ZD46; -.
DR   STRING; 272947.RP501; -.
DR   EnsemblBacteria; CAA14953; CAA14953; CAA14953.
DR   KEGG; rpr:RP501; -.
DR   PATRIC; fig|272947.5.peg.510; -.
DR   eggNOG; COG0130; Bacteria.
DR   HOGENOM; CLU_032087_2_2_5; -.
DR   OMA; YSVADAW; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   5: Uncertain;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..172
FT                   /note="Putative tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121895"
FT   ACT_SITE        39
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   172 AA;  19116 MW;  66B2F753BDA8566C CRC64;
     MGNYWLNFYK PRGISSAKLV NIVKKIIGKT KIGHAGTLDV EAEGILPLAV GEATKLIQLL
     IDARKTYIFS VKFGTQTDSG DYTGKVIASK DYIPSQEEVY TVCSKFIGNI RQVPPIFSAI
     KVNGVRAYKL AREGKIVELK PRNITIYDLK CLNFDKENAI ATYYTECSKG LI