ID   TRUB_SACS2              Reviewed;         251 AA.
AC   Q980C3;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Probable tRNA pseudouridine synthase B;
DE            EC=5.4.99.25;
DE   AltName: Full=tRNA pseudouridine(55) synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=truB; OrderedLocusNames=SSO0393;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
CC   -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE006641; AAK40720.1; -; Genomic_DNA.
DR   PIR; A99183; A99183.
DR   RefSeq; WP_009988804.1; NC_002754.1.
DR   AlphaFoldDB; Q980C3; -.
DR   SMR; Q980C3; -.
DR   STRING; 273057.SSO0393; -.
DR   EnsemblBacteria; AAK40720; AAK40720; SSO0393.
DR   GeneID; 44129372; -.
DR   KEGG; sso:SSO0393; -.
DR   PATRIC; fig|273057.12.peg.387; -.
DR   eggNOG; arCOG00987; Archaea.
DR   HOGENOM; CLU_032087_3_0_2; -.
DR   InParanoid; Q980C3; -.
DR   OMA; GPFKEDE; -.
DR   PhylomeDB; Q980C3; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000495; P:box H/ACA RNA 3'-end processing; IBA:GO_Central.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031118; P:rRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031120; P:snRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.130.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR   InterPro; IPR032819; TruB_C.
DR   InterPro; IPR004521; Uncharacterised_CHP00451.
DR   PANTHER; PTHR23127; PTHR23127; 1.
DR   Pfam; PF01472; PUA; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00425; CBF5; 1.
DR   TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..251
FT                   /note="Probable tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121971"
FT   DOMAIN          163..237
FT                   /note="PUA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
SQ   SEQUENCE   251 AA;  28668 MW;  FA184048749B5328 CRC64;
     MLPIGIENAT KIMNYISKGG KEYVCVMQVH CEYNKEELAK IISSFKGEIY QRPPVRSSVK
     RRLRIRRIYD IEILDMDKKL VLLRVNCDSG TYMRKLCHDI GIIYGCGAHM RELRRTRSGI
     FTESTNLVKL HELSEAIYLY KNCKDETELR RVLIPMEFAT CEIPKIVIED SAVNALAYGA
     QLAVPGVVAY QNFKKNDTVA VLTLKGELVA TGNALMDSEE LENAKKGIVV NLSRVFMQRD
     IYPKAWKKHE S