ID   TRUB_STRPN              Reviewed;         292 AA.
AC   Q97QJ3;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=SP_1212;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC   -!- INTERACTION:
CC       Q97QJ3; A0A0H2URH9: SP_1748; NbExp=4; IntAct=EBI-6473797, EBI-6473801;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR   EMBL; AE005672; AAK75319.1; -; Genomic_DNA.
DR   PIR; F95140; F95140.
DR   RefSeq; WP_001013240.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; Q97QJ3; -.
DR   SMR; Q97QJ3; -.
DR   IntAct; Q97QJ3; 1.
DR   STRING; 170187.SP_1212; -.
DR   EnsemblBacteria; AAK75319; AAK75319; SP_1212.
DR   KEGG; spn:SP_1212; -.
DR   eggNOG; COG0130; Bacteria.
DR   OMA; ELQFIRW; -.
DR   PhylomeDB; Q97QJ3; -.
DR   BioCyc; SPNE170187:G1FZB-1227-MON; -.
DR   BRENDA; 5.4.99.25; 1960.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   1: Evidence at protein level;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..292
FT                   /note="tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121915"
FT   ACT_SITE        38
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ   SEQUENCE   292 AA;  32271 MW;  B7A192469C4BC168 CRC64;
     MNGIINLKKE AGMTSHDAVF KLRKILGTKK IGHGGTLDPD VVGVLPIAVG KATRMVEFMQ
     DEGKIYEGEI TLGYSTKTED ASGEVVAETP VLSLLDEKLV DEAIASLTGP ITQIPPMYSA
     VKVNGRKLYE YARAGQEVER PERQVTIYQF ERTSPISYDG QLARFTFRVK CSKGTYIRTL
     SVDLGEKLGY AAHMSHLTRT SAAGLQLEDA LALEEIAEKV EAGQLDFLHP LEIGTGDLVK
     VFLSPEEATE VRFGRFIELD QTDKELAAFE DDKLLAILEK RGNLYKPRKV FS