ID   TRUB_SULTO              Reviewed;         337 AA.
AC   Q975L5; F9VMW9;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Probable tRNA pseudouridine synthase B;
DE            EC=5.4.99.25;
DE   AltName: Full=tRNA pseudouridine(55) synthase;
DE            Short=Psi55 synthase;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=truB; OrderedLocusNames=STK_04020;
OS   Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS   (Sulfolobus tokodaii).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfurisphaera.
OX   NCBI_TaxID=273063;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX   PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA   Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA   Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA   Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA   Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT   "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT   Sulfolobus tokodaii strain7.";
RL   DNA Res. 8:123-140(2001).
CC   -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BA000023; BAK54266.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q975L5; -.
DR   SMR; Q975L5; -.
DR   STRING; 273063.STK_04020; -.
DR   EnsemblBacteria; BAK54266; BAK54266; STK_04020.
DR   KEGG; sto:STK_04020; -.
DR   PATRIC; fig|273063.9.peg.464; -.
DR   eggNOG; arCOG00987; Archaea.
DR   OMA; GPFKEDE; -.
DR   Proteomes; UP000001015; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01081; TruB_arch; 1.
DR   InterPro; IPR012960; Dyskerin-like.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR   InterPro; IPR026326; TruB_arch.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR23127; PTHR23127; 1.
DR   Pfam; PF08068; DKCLD; 1.
DR   Pfam; PF01472; PUA; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SMART; SM01136; DKCLD; 1.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00425; CBF5; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..337
FT                   /note="Probable tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121972"
FT   DOMAIN          250..324
FT                   /note="PUA"
FT   ACT_SITE        83
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   337 AA;  38504 MW;  7251B3174132593E CRC64;
     MEIYDFIYKI DSFCNYSNNW NIIQDSYTNE KYGYFADKRP IEVLIKNSII NADKPPGPTS
     HEVAYWIKQM FKVSKAGHGG TLDPKVTGVL PIGLENATKL MSYISSSGKE YVCLMQVHCD
     FNIDELKQII SKFIGIIYQK PPVRSSVKRR TRKKKIYDIE ILDTDKRFIL LRISSDPGTY
     MRKLCHDIGV ILGCGAHMRE LRRIRSGIFT EKNLVTLQEI SEALYMWKNC KDESDLRKIL
     LPMEYATCGM PKILIDDNAV DAISYGAMLT APGIVAYQRF RVKDTVAILT LKGELVAIGE
     ADVDSQKLVD MKKGIVVKPK RVLMPRDIYP RSWKKHG