ID   TRUB_SYNE7              Reviewed;         294 AA.
AC   Q8KIY0; Q31NW7; Q9Z3H8;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 2.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01080};
GN   OrderedLocusNames=Synpcc7942_1222; ORFNames=sef0005;
OS   Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS   R2).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Holtman C.K., Sandoval P., Chen Y., Socias T., Mohler B.J., McMurtry S.,
RA   Gonzalez A., Salinas I., Golden S.S., Youderian P.;
RT   "Synechococcus elongatus PCC7942 cosmid 7G3.";
RL   Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-153.
RX   PubMed=9727980; DOI=10.1126/science.281.5382.1519;
RA   Ishiura M., Kutsuna S., Aoki S., Iwasaki H., Andersson C.R., Tanabe A.,
RA   Golden S.S., Johnson C.H., Kondo T.;
RT   "Expression of a gene cluster kaiABC as a circadian feedback process in
RT   cyanobacteria.";
RL   Science 281:1519-1523(1998).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC       the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
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DR   EMBL; AY120853; AAM82680.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB57252.1; -; Genomic_DNA.
DR   EMBL; AB010691; BAA37097.1; -; Genomic_DNA.
DR   PIR; T44263; T44263.
DR   RefSeq; WP_011242642.1; NC_007604.1.
DR   AlphaFoldDB; Q8KIY0; -.
DR   SMR; Q8KIY0; -.
DR   STRING; 1140.Synpcc7942_1222; -.
DR   PRIDE; Q8KIY0; -.
DR   EnsemblBacteria; ABB57252; ABB57252; Synpcc7942_1222.
DR   KEGG; syf:Synpcc7942_1222; -.
DR   eggNOG; COG0130; Bacteria.
DR   HOGENOM; CLU_032087_0_0_3; -.
DR   OMA; ELQFIRW; -.
DR   OrthoDB; 1166299at2; -.
DR   BioCyc; SYNEL:SYNPCC7942_1222-MON; -.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR   Gene3D; 2.30.130.10; -; 1.
DR   HAMAP; MF_01080; TruB_bact; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR   InterPro; IPR015240; tRNA_sdUridine_synth_fam1_C.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR13767; PTHR13767; 1.
DR   Pfam; PF09157; TruB-C_2; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00431; TruB; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..294
FT                   /note="tRNA pseudouridine synthase B"
FT                   /id="PRO_0000121926"
FT   ACT_SITE        40
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
FT   CONFLICT        158..187
FT                   /note="SGSQAELSVEIHCGAGTYIRSLARDLGAVL -> IGQSSRIICRNPLWCWHL
FT                   HPFSSARPRSRV (in Ref. 1; AAM82680)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   294 AA;  31539 MW;  7CED8E3BE9CDC7D8 CRC64;
     MSSEGFVNLD KPAGWTSHDC VAKLRRLLRE RRIGHGGTLD PAVTGVLPIA VGRATRLLPY
     LPSGKTYVGT IRFGLQTSTD DLTGDRLAEA DTSHLSREAI EAALPQFLGH IQQQPPQVSA
     VQVQGQRLYQ LARRGEAPTE LPWRTVEIQS IRILQWRSGS QAELSVEIHC GAGTYIRSLA
     RDLGAVLGVG GTLAELRRTA SSGFDLNQST PLTELLDGAA VPLLALDLPL QHLAKVQLSA
     ETSDRWRQGQ AMPVPDSVLP DAAPVRVYDL NGQFLGIGAI AAGLCKPKVV LAAL