TRUB_THEON
ID TRUB_THEON Reviewed; 334 AA.
AC B6YSP4;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2009, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01081};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01081};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01081}; OrderedLocusNames=TON_0096;
OS Thermococcus onnurineus (strain NA1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=523850;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NA1;
RX PubMed=18790866; DOI=10.1128/jb.00746-08;
RA Lee H.S., Kang S.G., Bae S.S., Lim J.K., Cho Y., Kim Y.J., Jeon J.H.,
RA Cha S.-S., Kwon K.K., Kim H.-T., Park C.-J., Lee H.-W., Kim S.I., Chun J.,
RA Colwell R.R., Kim S.-J., Lee J.-H.;
RT "The complete genome sequence of Thermococcus onnurineus NA1 reveals a
RT mixed heterotrophic and carboxydotrophic metabolism.";
RL J. Bacteriol. 190:7491-7499(2008).
CC -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC Rule:MF_01081}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01081};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01081}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000855; ACJ15581.1; -; Genomic_DNA.
DR RefSeq; WP_012571054.1; NC_011529.1.
DR AlphaFoldDB; B6YSP4; -.
DR SMR; B6YSP4; -.
DR STRING; 523850.TON_0096; -.
DR EnsemblBacteria; ACJ15581; ACJ15581; TON_0096.
DR GeneID; 7017743; -.
DR KEGG; ton:TON_0096; -.
DR PATRIC; fig|523850.10.peg.96; -.
DR eggNOG; arCOG00987; Archaea.
DR HOGENOM; CLU_032087_3_0_2; -.
DR OMA; GPFKEDE; -.
DR OrthoDB; 36028at2157; -.
DR Proteomes; UP000002727; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.30.130.10; -; 1.
DR HAMAP; MF_01081; TruB_arch; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR026326; TruB_arch.
DR InterPro; IPR032819; TruB_C.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR23127; PTHR23127; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 2.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00425; CBF5; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 3: Inferred from homology;
KW Isomerase; tRNA processing.
FT CHAIN 1..334
FT /note="Probable tRNA pseudouridine synthase B"
FT /id="PRO_1000136826"
FT DOMAIN 250..325
FT /note="PUA"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT ACT_SITE 82
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
SQ SEQUENCE 334 AA; 37749 MW; DD56EF1037216F12 CRC64;
MARDEVRRIL PADIKREVLI KDEKAETNPK WGFPPEKRPM EMHMQFGIIN LDKPPGPTSH
EVVAWVKKLF NLNKAGHGGT LDPKVSGVLP VALERATRVV QALLPAGKEY VALMHLHGEV
PEDKIYAVMK EFEGEIIQRP PLRSAVKRRL RTRKVYYIEI LEIDGKDVLF RVGVEAGTYI
RSLIHHIGLA LGVGAHMAEL RRTRSGPFKE DETLVTLHDL VDYYHFWKED GIEEYFRKAI
QPMEKAVEHL PKVWIRDSAV AAVTHGADLA VPGIVKVHKG IKKGDLVAVM TLKDELVALG
KATMTSGEML QKSKGIAVDV DKVFMPRDWY PKLW