TRUB_THET8
ID TRUB_THET8 Reviewed; 312 AA.
AC Q5SLS6;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01080};
DE EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01080};
DE AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01080};
GN Name=truB {ECO:0000255|HAMAP-Rule:MF_01080}; OrderedLocusNames=TTHA0217;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01080};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP008226; BAD70040.1; -; Genomic_DNA.
DR RefSeq; WP_011174127.1; NC_006461.1.
DR RefSeq; YP_143483.1; NC_006461.1.
DR AlphaFoldDB; Q5SLS6; -.
DR SMR; Q5SLS6; -.
DR STRING; 300852.55771599; -.
DR EnsemblBacteria; BAD70040; BAD70040; BAD70040.
DR GeneID; 3170140; -.
DR KEGG; ttj:TTHA0217; -.
DR PATRIC; fig|300852.9.peg.215; -.
DR eggNOG; COG0130; Bacteria.
DR HOGENOM; CLU_032087_0_2_0; -.
DR OMA; ELQFIRW; -.
DR PhylomeDB; Q5SLS6; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR CDD; cd02573; PseudoU_synth_EcTruB; 1.
DR HAMAP; MF_01080; TruB_bact; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR014780; tRNA_psdUridine_synth_TruB.
DR PANTHER; PTHR13767; PTHR13767; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00431; TruB; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..312
FT /note="tRNA pseudouridine synthase B"
FT /id="PRO_0000121931"
FT ACT_SITE 37
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01080"
SQ SEQUENCE 312 AA; 34292 MW; 373609E637B1BC19 CRC64;
MALYAVDKPL HLTSHDVVEE ARRRLSTRRV GHTGTLDPLA TGLLLLVTNE STKLVPFLSG
EDKEYIAWVS FGATTPTLDA EGPISEEAPA RFDRKDLEAA LPRFLEVREQ VPPLYSAIKV
GGKRAYEAAR EGKPLALGPR PVRYLEVELL AFDPEPIPHP IAPSARGWRL AERRGRPVRL
PRPLGAYPTA VVRLVVGPGT YVRAFARDLG EMLGTKAFLS GLVRTRVGRV GLERAVALSD
LSPEKAIPEL DVLPFPVVEL SHTEARRVLE GMPLPIPAMG YVTLVDSKRR LLAIAEGDGF
KLKIRRVFAK EA
