ID   TRUC_ECOL6              Reviewed;         260 AA.
AC   Q8FEF9;
DT   09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=tRNA pseudouridine synthase C;
DE            EC=5.4.99.26;
DE   AltName: Full=tRNA pseudouridine(65) synthase;
DE   AltName: Full=tRNA pseudouridylate synthase C;
DE   AltName: Full=tRNA-uridine isomerase C;
GN   Name=truC; OrderedLocusNames=c3357;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-65 in
CC       transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(65) in tRNA = pseudouridine(65) in tRNA;
CC         Xref=Rhea:RHEA:42536, Rhea:RHEA-COMP:10103, Rhea:RHEA-COMP:10104,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.26;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE014075; AAN81804.1; -; Genomic_DNA.
DR   RefSeq; WP_000889992.1; NC_004431.1.
DR   AlphaFoldDB; Q8FEF9; -.
DR   SMR; Q8FEF9; -.
DR   STRING; 199310.c3357; -.
DR   EnsemblBacteria; AAN81804; AAN81804; c3357.
DR   KEGG; ecc:c3357; -.
DR   eggNOG; COG0564; Bacteria.
DR   HOGENOM; CLU_016902_11_4_6; -.
DR   OMA; DRHETQF; -.
DR   BioCyc; ECOL199310:C3357-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..260
FT                   /note="tRNA pseudouridine synthase C"
FT                   /id="PRO_0000162712"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   260 AA;  29608 MW;  0BF4B3F20343D9FD CRC64;
     MLEILYQDEW LVAVNKPSGW LVHRSWLDRD EKVVVMQTVR DQIGQHVFTA HRLDRPTSGV
     LLMGLSSEAG RLLAQQFEQH QIQKRYHAIV RGWLMEEAVL DYPLAEELDK IADKFAREDK
     GPQPAVTHYR GLATVEMPVA TGRYPTTRYG LVELEPKTGR KHQLRRHLAH LRHPIIGDSK
     HGDLRQNRSG AEHFGLQRLM LHASQLSLTH PFTGEPLAIH AGLDDTWMQA LSQFGWRGLL
     PENERVEFSA PSGQDGEISS