TRUC_ECOLI
ID TRUC_ECOLI Reviewed; 260 AA.
AC P0AA41; Q2MA43; Q46918;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=tRNA pseudouridine synthase C;
DE EC=5.4.99.26;
DE AltName: Full=tRNA pseudouridine(65) synthase;
DE AltName: Full=tRNA pseudouridylate synthase C;
DE AltName: Full=tRNA-uridine isomerase C;
GN Name=truC; Synonyms=yqcB; OrderedLocusNames=b2791, JW2762;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF ASP-54.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=11720289;
RA Del Campo M., Kaya Y., Ofengand J.;
RT "Identification and site of action of the remaining four putative
RT pseudouridine synthases in Escherichia coli.";
RL RNA 7:1603-1615(2001).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-65 in
CC transfer RNAs. {ECO:0000269|PubMed:11720289}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(65) in tRNA = pseudouridine(65) in tRNA;
CC Xref=Rhea:RHEA:42536, Rhea:RHEA-COMP:10103, Rhea:RHEA-COMP:10104,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.26;
CC Evidence={ECO:0000269|PubMed:11720289};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U29581; AAB40441.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75833.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76863.1; -; Genomic_DNA.
DR PIR; C65061; C65061.
DR RefSeq; NP_417271.1; NC_000913.3.
DR RefSeq; WP_000890001.1; NZ_SSUR01000022.1.
DR AlphaFoldDB; P0AA41; -.
DR SMR; P0AA41; -.
DR BioGRID; 4262247; 15.
DR BioGRID; 851572; 1.
DR IntAct; P0AA41; 1.
DR STRING; 511145.b2791; -.
DR jPOST; P0AA41; -.
DR PaxDb; P0AA41; -.
DR PRIDE; P0AA41; -.
DR EnsemblBacteria; AAC75833; AAC75833; b2791.
DR EnsemblBacteria; BAE76863; BAE76863; BAE76863.
DR GeneID; 67413934; -.
DR GeneID; 947242; -.
DR KEGG; ecj:JW2762; -.
DR KEGG; eco:b2791; -.
DR PATRIC; fig|1411691.4.peg.3942; -.
DR EchoBASE; EB2963; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_11_4_6; -.
DR InParanoid; P0AA41; -.
DR OMA; DRHETQF; -.
DR PhylomeDB; P0AA41; -.
DR BioCyc; EcoCyc:G7449-MON; -.
DR BioCyc; MetaCyc:G7449-MON; -.
DR PRO; PR:P0AA41; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0009982; F:pseudouridine synthase activity; IMP:EcoliWiki.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IMP:EcoCyc.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0009451; P:RNA modification; IMP:EcoliWiki.
DR GO; GO:0006399; P:tRNA metabolic process; IMP:EcoliWiki.
DR GO; GO:0008033; P:tRNA processing; IMP:EcoliWiki.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IMP:EcoCyc.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..260
FT /note="tRNA pseudouridine synthase C"
FT /id="PRO_0000162711"
FT ACT_SITE 54
FT MUTAGEN 54
FT /note="D->T: Loss of activity."
FT /evidence="ECO:0000269|PubMed:11720289"
SQ SEQUENCE 260 AA; 29666 MW; 0BEA421482CDB89A CRC64;
MLEILYQDEW LVAVNKPSGW LVHRSWLDRD EKVVVMQTVR DQIGQHVFTA HRLDRPTSGV
LLMGLSSEAG RLLAQQFEQH QIQKRYHAIV RGWLMEEAVL DYPLVEELDK IADKFAREDK
GPQPAVTHYR GLATVEMPVA TGRYPTTRYG LVELEPKTGR KHQLRRHLAH LRHPIIGDSK
HGDLRQNRSG AEHFGLQRLM LHASQLSLTH PFTGEPLTIH AGLDDTWMQA LSQFGWRGLL
PENERVEFSA PSGQDGEISS
